Laboratory of Histology, Embryology, and Cytogenetic, Faculty of Medicine and Pharmacy, Mohammed V University in Rabat, 10100 Rabat, Morocco.
Center of Data Science and Information Technology, INTI International University, 71800 Nilai, Malaysia.
Front Biosci (Landmark Ed). 2023 Sep 27;28(9):229. doi: 10.31083/j.fbl2809229.
Screening new natural molecules with pharmacological and/or cosmetic properties remains a highly sought-after area of research. Moreover, essential oils and volatile compounds have recently garnered significant interest as natural substance candidates. In this study, the volatile components of L. essential oils (PLEOs) isolated from the fruit and its main compounds, alpha-pinene, and limonene, are investigated for antioxidant, antidiabetic, and dermatoprotective activities.
antioxidant activity was investigated using 2,2'-diphenyl-1-picrylhydrazyl (DPPH), fluorescence recovery after photobleaching (FRAP), and 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) methods. The antidiabetic and dermatoprotective effects were studied using enzyme inhibitory activities.
Antioxidant tests showed that PLEO has the best activity (ranging from 29.64 ± 3.04 to 73.80 ± 3.96 µg/mL) compared to its main selected molecules (ranging from 74 ± 3.72 to 107.23 ± 5.03 µg/mL). The α-glucosidase and α-amylase assays demonstrated that the elements tested have a promising antidiabetic potential with IC50values ranging from 78.03 ± 2.31 to 116.03 ± 7.42 µg/mL and 74.39 ± 3.08 to 112.35 ± 4.92 µg/mL for the α-glucosidase and α-amylase assays, respectively, compared to the standard drug. For the tyrosinase test, we found that the EOs (IC50 = 57.72 ± 2.86 µg/mL) followed by limonene (IC50 = 74.24 ± 2.06 µg/mL) and α-pinene (IC50 = 97.45 ± 5.22 µg/mL) all exhibited greater inhibitory effects than quercetin (IC50 = 246.90 ± 2.54 µg/mL).
Our results suggest that the biological activities of PLEO, as well as its main compounds, make them promising candidates for the development of new strategies aimed at improving dermatoprotection and treating diseases associated with diabetes mellitus and oxidative stress.
筛选具有药理和/或美容特性的新型天然分子仍然是一个备受关注的研究领域。此外，精油和挥发性化合物最近作为天然物质候选物引起了极大的兴趣。在这项研究中，研究了从果实中分离出的 L. 精油（PLEO）及其主要化合物α-蒎烯和柠檬烯的挥发性成分的抗氧化、抗糖尿病和皮肤保护活性。
使用 2,2'-二苯基-1-苦基肼（DPPH）、光漂白后荧光恢复（FRAP）和 2,2-氮杂双-3-乙基苯并噻唑啉-6-磺酸（ABTS）方法研究抗氧化活性。使用酶抑制活性研究抗糖尿病和皮肤保护作用。
抗氧化测试表明，与主要选择的分子（范围为 74 ± 3.72 至 107.23 ± 5.03 µg/mL）相比，PLEO 具有最佳活性（范围为 29.64 ± 3.04 至 73.80 ± 3.96 µg/mL）。α-葡萄糖苷酶和α-淀粉酶测定表明，测试的元素具有有前途的抗糖尿病潜力，IC50 值范围分别为 78.03 ± 2.31 至 116.03 ± 7.42 µg/mL 和 74.39 ± 3.08 至 112.35 ± 4.92 µg/mL，对于α-葡萄糖苷酶和α-淀粉酶测定，与标准药物相比。对于酪氨酸酶试验，我们发现 EOs（IC50 = 57.72 ± 2.86 µg/mL）紧随其后的是柠檬烯（IC50 = 74.24 ± 2.06 µg/mL）和α-蒎烯（IC50 = 97.45 ± 5.22 µg/mL）均表现出比槲皮素（IC50 = 246.90 ± 2.54 µg/mL）更强的抑制作用。
我们的结果表明，PLEO 及其主要化合物的生物学活性使它们成为开发旨在改善皮肤保护和治疗与糖尿病和氧化应激相关疾病的新策略的有希望的候选物。
