Department of Chemistry and Biochemistry, University of Mississippi, University, Mississippi, USA.
Department of Chemistry, Faculty of Agriculture, Damietta University, Damietta, Egypt.
Cannabis Cannabinoid Res. 2024 Aug;9(4):e1091-e1107. doi: 10.1089/can.2022.0335. Epub 2023 Oct 5.
is a psychoactive plant indigenous to Central and South Asia, traditionally used both for recreational and religious purposes, in addition to folk medicine. Cannabis is a rich source of natural compounds, the most important of which are commonly known as cannabinoids that cause a variety of effects through interaction with the endocannabinoid system. In this study, a high-performance liquid chromatography-ultraviolet/photodiode array (PDA) method was developed and validated for the analysis of 15 cannabinoids in cannabis plant materials and cannabis-based marketed products. These cannabinoids are cannabidivarinic acid, cannabidivarin, cannabidiolic acid, cannabigerolic acid, cannabigerol, cannabidiol, delta-9-tetrahydrocannabivarin, delta-9-tetrahydrocannabivarinic acid, cannabinol, delta-9-tetrahyrocannabinol, delta-8-tetrahyrocannabinol, cannabicyclol, cannabichromene, delta-9-tetrahyrocannabinolic acid A, and cannabichromenic acid. The separation was carried out using a reversed-phase Luna C18(2) column and a mobile phase consisting of 75% acetonitrile and 0.1% formic acid in water. A PDA detector was used, and data were extracted at =220 nm. Principal component analysis of cannabis four varieties was performed. The method was linear over the calibration range of 5-75 μg/mL with >0.999 for all cannabinoids. This method was sensitive and gave good baseline separation of all examined cannabinoids with limits of detection ranging between 0.2 and 1.6 μg/mL and limits of quantification ranging between 0.6 and 4.8 μg/mL. The average recoveries for all cannabinoids were between 81% and 104%. The measured repeatability and intermediate precisions (% relative standard deviation) in all varieties ranged from 0.35% to 9.84% and 1.11% to 5.26%, respectively. The proposed method is sensitive, selective, reproducible, and accurate. It can be applied for the simultaneous determination of these cannabinoids in the biomass and cannabis-derived marketed products.
大麻是一种原产于中亚和南亚的精神活性植物，传统上除了用于民间医学外，还被用于娱乐和宗教目的。大麻是天然化合物的丰富来源，其中最重要的是通常被称为大麻素的化合物，它们通过与内源性大麻素系统相互作用引起各种影响。 在这项研究中，开发并验证了一种高效液相色谱-紫外/光电二极管阵列（PDA）方法，用于分析大麻植物材料和基于大麻的市售产品中的 15 种大麻素。这些大麻素是大麻二酚酸、大麻二酚、大麻二酚酸、大麻萜酚酸、大麻萜酚、大麻二酚、Δ9-四氢大麻酚、Δ9-四氢大麻酚酸、大麻酚、Δ9-四氢大麻醇、Δ8-四氢大麻醇、大麻环醇、大麻色烯、Δ9-四氢大麻酚酸 A 和大麻色烯酸。分离是在反相 Luna C18(2)柱上进行的，流动相由 75%乙腈和 0.1%甲酸在水中组成。使用 PDA 检测器，在 =220nm 处提取数据。对大麻的四个品种进行了主成分分析。 该方法在 5-75μg/mL 的校准范围内呈线性，所有大麻素的相关系数均大于 0.999。该方法灵敏，可对所有检查的大麻素进行良好的基线分离，检测限范围在 0.2 至 1.6μg/mL 之间，定量限范围在 0.6 至 4.8μg/mL 之间。所有大麻素的平均回收率在 81%至 104%之间。在所有品种中，测量的重复性和中间精密度（相对标准偏差%）的范围分别为 0.35%至 9.84%和 1.11%至 5.26%。 所提出的方法灵敏、选择性好、重现性和准确性高。它可用于同时测定生物质和大麻衍生的市售产品中的这些大麻素。
