家庭真菌多样性与纽约市学龄儿童哮喘发病的关系。

Fungal diversity in homes and asthma morbidity among school-age children in New York City.

机构信息

Division of Pulmonary, Critical Care, and Sleep Medicine, College of Medicine, Ohio State University, Columbus, OH, 43210, USA.

Department of Environmental Health Sciences, Mailman School of Public Health, Columbia University, New York, NY, 10032, USA.

出版信息

Environ Res. 2023 Dec 15;239(Pt 1):117296. doi: 10.1016/j.envres.2023.117296. Epub 2023 Oct 6.

DOI:10.1016/j.envres.2023.117296

PMID:37806477

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10842248/

Abstract

BACKGROUND

Asthma development has been inversely associated with exposure to fungal diversity. However, the influence of fungi on measures of asthma morbidity is not well understood.

OBJECTIVES

This study aimed to test the hypothesis that fungal diversity is inversely associated with neighborhood asthma prevalence and identify specific fungal species associated with asthma morbidity.

METHODS

Children aged 7-8 years (n = 347) living in higher (11-18%) and lower (3-9%) asthma prevalence neighborhoods were recruited within an asthma case-control study. Fungal communities were analyzed from floor dust using high-throughput DNA sequencing. A subset of asthmatic children (n = 140) was followed to age 10-11 to determine asthma persistence.

RESULTS

Neighborhood asthma prevalence was inversely associated with fungal species richness (P = 0.010) and Shannon diversity (P = 0.059). Associations between neighborhood asthma prevalence and diversity indices were driven by differences in building type and presence of bedroom carpet. Among children with asthma at age 7-8 years, Shannon fungal diversity was inversely associated with frequent asthma symptoms at that age (OR 0.57, P = 0.025) and with asthma persistence to age 10-11 (OR 0.48, P = 0.043). Analyses of individual fungal species did not show significant associations with asthma outcomes when adjusted for false discovery rates.

DISCUSSION

Lower fungal diversity was associated with asthma symptoms in this urban setting. Individual fungal species associated with asthma morbidity were not detected. Further research is warranted into building type, carpeting, and other environmental characteristics which influence fungal exposures in homes.

摘要

背景

哮喘的发展与真菌多样性的暴露呈负相关。然而，真菌对哮喘发病率的影响尚不清楚。

目的

本研究旨在检验真菌多样性与社区哮喘患病率呈负相关的假设，并确定与哮喘发病率相关的特定真菌物种。

方法

在一项哮喘病例对照研究中，招募了居住在哮喘高患病率（11-18%）和低患病率（3-9%）社区的 7-8 岁儿童（n=347）。使用高通量 DNA 测序分析地板灰尘中的真菌群落。对一部分哮喘儿童（n=140）进行了随访，随访至 10-11 岁，以确定哮喘的持续情况。

结果

社区哮喘患病率与真菌物种丰富度（P=0.010）和 Shannon 多样性（P=0.059）呈负相关。社区哮喘患病率与多样性指数之间的关联是由建筑类型和卧室地毯的存在差异驱动的。在 7-8 岁时患有哮喘的儿童中，Shannon 真菌多样性与该年龄时频繁的哮喘症状呈负相关（OR 0.57，P=0.025），与哮喘持续到 10-11 岁（OR 0.48，P=0.043）也呈负相关。在调整假发现率后，分析单个真菌物种与哮喘结局之间没有显示出显著关联。