Balansky R M, Blagoeva P M, Mircheva Z I, Stoitchev I, Chernozemskí I
J Cancer Res Clin Oncol. 1986;112(3):272-5. doi: 10.1007/BF00395922.
The effect of vitamins A, C and E, butylated hydroxytoluene (BHT) and glutathione (GSH) on gastric carcinogenesis induced by N-methyl-N-nitro-N-nitrosoguanidine (MNNG) was investigated. Male and female BD-VI rats 2-3 months old received a single oral application of MNNG dissolved in corn oil. The male rats were divided into four groups: Group-I: MNNG 250 mg/kg by intubation; Group-II: MNNG + vitamin C daily in the drinking water (400 mg/l); Group-III: MNNG + vitamin C (400 mg/l) + 100 g of milk broth (for each of 10 rats) containing vitamin A (40,000 IU), vitamin E (0.5 g) and BHT (0.1 g) three times a week. The treatment with antioxidants started 7 days before the MNNG administration and continued until the end of experiment. Group-IV rats received MNNG + oxyferriscorbone, i.p. as a single dose of 1.0 mg/kg, daily during the week before and the week after MNNG exposure and than 3 times a week till the end of the experiment. Female rats were divided into two groups: Group-I: MNNG 333 mg/kg by intubation; Group-II: MNNG + GSH orally at a dose of 100 mg/rat 1 h before and 5, 24, 48, and 72 h after MNNG intubation. The incidence of gastric tumors after 15 months of treatment was as follows: male rats, 82.4% in Group-I, 40.0% in Group-II, 40.7% in Group-III, and 50.0% in Group-IV; female rats; 72.7% in Group-I, and 36.0% in Group-II.
研究了维生素A、C和E、丁基羟基甲苯（BHT）和谷胱甘肽（GSH）对N-甲基-N-硝基-N-亚硝基胍（MNNG）诱导的胃癌发生的影响。2至3个月大的雄性和雌性BD-VI大鼠经口单次给予溶于玉米油的MNNG。雄性大鼠分为四组：第一组：经插管给予MNNG 250 mg/kg；第二组：饮用水中每日添加维生素C（400 mg/l）+MNNG；第三组：饮用水中添加维生素C（400 mg/l）+MNNG+每周三次给10只大鼠每只喂100 g含维生素A（40,000 IU）、维生素E（0.5 g）和BHT（0.1 g）的牛奶肉汤；抗氧化剂处理在给予MNNG前7天开始，持续至实验结束。第四组大鼠在MNNG暴露前一周和暴露后一周每天腹腔注射1.0 mg/kg的氧铁骨化醇，之后每周三次直至实验结束。雌性大鼠分为两组：第一组：经插管给予MNNG 333 mg/kg；第二组：在MNNG插管前1小时及插管后5、24、48和72小时经口给予每只大鼠100 mg GSH+MNNG。治疗15个月后的胃肿瘤发生率如下：雄性大鼠，第一组为82.4%，第二组为40.0%，第三组为40.7%，第四组为50.0%；雌性大鼠，第一组为72.7%，第二组为36.0%。
