Department of Applied Optics and Photonics, University of Calcutta, JD-2, Sector-III, Salt Lake, Kolkata, West Bengal, 700 106, India.
Department of Chemical and Biological Sciences, S. N. Bose National Centre for Basic Sciences, Kolkata, 700106, India.
Sci Rep. 2023 Oct 12;13(1):17306. doi: 10.1038/s41598-023-44011-y.
The recent prediction of diabetes to be a global pandemic invites a detection strategy preferably non-invasive, and bloodless to manage the disease and the associated complications. Here, we have synthesized chitosan polymer functionalized, organic-inorganic bio-compatible nano-hybrids of MnO nanoparticles, and characterized it by utilizing several optical methodologies for the structural characterization which shows the Michaelis Menten (MM) kinetics for glucose and alpha-amylase protein (well-known diabetes biomarkers). We have also studied the potentiality for the detection of alpha-amylase in human salivary secretion which is reported to be strongly correlated with uncontrolled hyperglycemia. Finally, we have developed a prototype for the measurement of glucose (LOD of 0.38 mg/dL, LOQ of 1.15 mg/dL) and HbA1c (LOD of 0.15% and LOQ of 0.45%) utilizing the basic knowledge in the study for the detection of uncontrolled hyperglycemia at the point-of-care. With the limited number of clinical trials, we have explored the potential of our work in combating the diabetic pandemic across the globe in near future.
最近有预测称糖尿病将成为一种全球性流行病，因此需要一种优选非侵入性、无血的检测策略来控制这种疾病及其相关并发症。在这里，我们合成了壳聚糖聚合物功能化的 MnO 纳米粒子的有机-无机生物相容性纳米杂化物，并利用多种光学方法对其进行了结构表征，结果表明其具有葡萄糖和α-淀粉酶蛋白（著名的糖尿病生物标志物）的米氏动力学。我们还研究了检测人唾液分泌中α-淀粉酶的潜力，因为唾液分泌中的α-淀粉酶与不受控制的高血糖密切相关。最后，我们利用在检测即时点不受控制的高血糖方面的研究知识，开发了一种用于测量葡萄糖（LOD 为 0.38 mg/dL，LOQ 为 1.15 mg/dL）和 HbA1c（LOD 为 0.15%，LOQ 为 0.45%）的原型设备。由于临床试验数量有限，我们探索了我们的工作在未来应对全球糖尿病流行方面的潜力。
