Khophloiklang Vassakorn, Chanapiwat Panida, Aunpad Ratchaneewan, Kaeoket Kampon
Semen Laboratory, Department of Clinical Sciences and Public Health, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom 73170, Thailand.
Faculty of Veterinary Science, Rajamangala University of Technology Srivijaya, Nakhon Si Thammarat 80240, Thailand.
Animals (Basel). 2023 Sep 27;13(19):3040. doi: 10.3390/ani13193040.
Boar sperm is sensitive to particular conditions during cryopreservation, resulting in an extreme reduction in fertilizing ability due to damage to the sperm membranes. PKMPH contains bioactive peptides that have antioxidant and antimicrobial activities. There is no information on the use of palm-kernel-meal-derived bioactive peptides for boar semen cryopreservation. This study aimed to examine the effects of bioactive peptides from PKMPH on post-thawed boar sperm quality. Boar semen ejaculates ( = 17) were collected and divided into six equal aliquots based on PKMPH concentrations (0, 1.25, 2.5, 5, 10, and 15 µg/mL) in a freezing extender. Semen samples were processed and cryopreserved using the liquid nitrogen vapor method. Thereafter, the frozen semen samples were thawed at 50 °C for 12 s and evaluated for sperm motility using a computer-assisted sperm analyzer and for sperm viability, acrosome integrity, mitochondrial function, and lipid peroxidation by measuring the level of malondialdehyde (MDA). The results demonstrate that the supplementation of PKMPH with 2.5 µg/mL afforded superior post-thawed sperm qualities, such as increased total motility, viability, acrosome integrity, and mitochondrial function by 10.7%, 12.3%, 18.3%, and 12.7%, respectively, when compared to the control group. PKMPH at a concentration of 2.5 µg/mL showed the lowest level of MDA (40.6 ± 2.0 µMol/L) compared to the other groups. In conclusion, adding PKMPH peptides at 2.5 µg/mL to the freezing extender reduced the oxidative damage associated with cryopreservation and resulted in higher post-thawed sperm quality.
公猪精子在冷冻保存期间对特定条件敏感，由于精子膜受损，导致受精能力极度下降。棕榈仁粕水解物（PKMPH）含有具有抗氧化和抗菌活性的生物活性肽。目前尚无关于棕榈仁粕衍生的生物活性肽用于公猪精液冷冻保存的相关信息。本研究旨在探讨PKMPH中的生物活性肽对解冻后公猪精子质量的影响。采集公猪射精精液（ = 17份），并根据冷冻稀释液中PKMPH的浓度（0、1.25、2.5、5、10和15 µg/mL）将其分成六个相等的等分试样。精液样本经过处理后，采用液氮蒸汽法进行冷冻保存。之后，将冷冻的精液样本在50℃解冻12秒，并使用计算机辅助精子分析仪评估精子活力，通过测量丙二醛（MDA）水平评估精子活力、顶体完整性、线粒体功能和脂质过氧化。结果表明，与对照组相比，添加2.5 µg/mL的PKMPH可使解冻后精子质量更优，如总活力、活力、顶体完整性和线粒体功能分别提高10.7%、12.3%、18.3%和12.7%。与其他组相比，浓度为2.5 µg/mL的PKMPH显示出最低水平的MDA（40.6 ± 2.0 µMol/L）。总之，在冷冻稀释液中添加2.5 µg/mL的PKMPH肽可减少与冷冻保存相关的氧化损伤，并提高解冻后精子质量。
