PD-L1 相关 IncRNAs 与胶质瘤的恶性特征和免疫微环境有关。

PD-L1-related IncRNAs are associated with malignant characteristics and immune microenvironment in glioma.

机构信息

Department of Neurology, Hunan Aerospace Hospital, Changsha 410205, Hunan, P.R. China.

Department of Neurology, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, P.R. China.

出版信息

Aging (Albany NY). 2023 Oct 12;15(19):10785-10810. doi: 10.18632/aging.205120.

DOI:10.18632/aging.205120

PMID:37837543

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10599717/

Abstract

BACKGROUND

The expression of long non-coding RNA (lncRNA) can function as diagnostic and therapeutic biomarker for tumors. This research explores the role of PD-L1-related lncRNAs in affecting malignant characteristics and the immune microenvironment of glioma.

METHODS

Downloading gene expression profiles and clinicopathological information of glioma from TCGA and CGGA databases, 6 PD-L1-related lncRNAs were identified through correlation analysis, Cox and LASSO regression analysis, establishing the risk score model based on them. Bioinformatics analysis and cell experiments were adopted to verify the effects of LINC01271 on glioma.

RESULTS

Risk scores based on 6 PD-L1-related lncRNAs (AL355974.3, LINC01271, AC011899.3, MIR4500HG, LINC02594, AL357055.3) can reflect malignant characteristics and immunotherapy response of glioma. Patients with high LINC01271 expression had a worse prognosis, a higher abundance of M1 subtype macrophages in the immune microenvironment, and a higher degree of tumor malignancy. Experiments confirmed its positive regulatory effect on the proliferation and migration of glioma cells.

CONCLUSIONS

The risk score model based on 6 PD-L1-related lncRNAs can reflect the malignant characteristics and prognosis of glioma. LINC01271 can independently be used as a new target for prognosis evaluation and therapy.

摘要

背景

长链非编码 RNA（lncRNA）的表达可以作为肿瘤的诊断和治疗生物标志物。本研究探讨了 PD-L1 相关 lncRNAs 在影响胶质瘤恶性特征和免疫微环境中的作用。

方法

从 TCGA 和 CGGA 数据库中下载胶质瘤的基因表达谱和临床病理信息，通过相关性分析、Cox 和 LASSO 回归分析，确定了 6 个与 PD-L1 相关的 lncRNAs，基于它们建立风险评分模型。采用生物信息学分析和细胞实验验证 LINC01271 对胶质瘤的影响。

结果

基于 6 个 PD-L1 相关 lncRNAs（AL355974.3、LINC01271、AC011899.3、MIR4500HG、LINC02594、AL357055.3）的风险评分可以反映胶质瘤的恶性特征和免疫治疗反应。高 LINC01271 表达的患者预后较差，免疫微环境中 M1 亚型巨噬细胞丰度较高，肿瘤恶性程度较高。实验证实其对胶质瘤细胞的增殖和迁移具有正向调节作用。