College of Animal Science and Veterinary Medicine, Jinzhou Medical University, Jinzhou 121001, China.
Liaoning Kaiwei Biotechnology Co., Ltd., Jinzhou 121000, China.
Poult Sci. 2023 Dec;102(12):103140. doi: 10.1016/j.psj.2023.103140. Epub 2023 Sep 22.
In this study, 2 types of drinking water were provided to broiler chicks to evaluate the relationship between the bacterial load of drinking water and cecal microbiota. One type of drinking water was untreated, while the other type was daily treated with sodium dichlorocyanurate (50 mg/L). A total of 240 broiler chicks were divided into 2 groups based on their initial body weight. There were 6 replicates in each group, and each replicate cage contained 20 birds. Each cage was assigned to a different floor of the battery cage. On the final day, water samples were collected from each replicate cage at the opening of the drinking cup height, and one bird was selected from each replicate cage to obtain cecal content samples for measuring microbiota composition using the 16S rRNA technique. We found that drinking water treated with sodium dichlorocyanurate significantly reduced the richness and diversity of microbiota and diminished/disappeared most gram-negative bacteria. Broiler chicks that consumed chlorinated drinking water exhibited changes in the composition of cecal microbiota, with Alistipes serving as the marker species in the cecal content of broiler chicks that consumed untreated water, whereas AF12 served as the marker species in the cecal content of broiler chicks that consumed chlorinated drinking water. Functional prediction using the MetaCyc database and species composition analysis of metabolic pathways showed that changes in 7 metabolic pathways were related to the abundance of Providencia. Therefore, we concluded that chlorinated drinking water reduced the bacterial load in drinking water, thereby altering the cecal microbiota composition and regulating the metabolic activity of broiler chicks.
在这项研究中，我们向肉鸡雏鸡提供了 2 种饮用水，以评估饮用水中细菌负荷与盲肠微生物群之间的关系。一种饮用水未经处理，另一种则每天用二氯异氰尿酸钠（50mg/L）处理。共有 240 只肉鸡雏鸡根据初始体重分为 2 组。每组有 6 个重复，每个重复笼中有 20 只鸡。每个笼被分配到鸡笼的不同楼层。在最后一天，从每个重复笼的饮水杯开口处收集水样，并从每个重复笼中选择 1 只鸡，以 16S rRNA 技术测量盲肠内容物样本中的微生物群落组成。我们发现，用二氯异氰尿酸钠处理的饮用水显著降低了微生物群的丰富度和多样性，并使大多数革兰氏阴性菌减少/消失。饮用氯化水的肉鸡雏鸡的盲肠微生物群落组成发生了变化，Alistipes 是饮用未处理水的肉鸡雏鸡盲肠内容物中的标记种，而 AF12 是饮用氯化水的肉鸡雏鸡盲肠内容物中的标记种。使用 MetaCyc 数据库进行功能预测和代谢途径的物种组成分析表明，7 条代谢途径的变化与普罗维登斯菌的丰度有关。因此，我们得出结论，氯化饮用水降低了饮用水中的细菌负荷，从而改变了盲肠微生物群的组成，并调节了肉鸡雏鸡的代谢活性。
