Department of Botany and Plant Pathology, Oregon State University, Cordley Hall, 2701 SW Campus Way, Corvallis, OR, 97333, USA.
USDA-ARS, National Germplasm Resources Laboratory, Beltsville, MD, 20705, USA.
Arch Virol. 2023 Oct 16;168(11):273. doi: 10.1007/s00705-023-05901-8.
The complete genome sequence of a new member of the family Mitoviridae was obtained from walking iris (Trimezia northiana (Schneev.) Ravenna by high-throughput sequencing. This is the first putative mitovirus identified in a monocotyledonous plant. The new mitovirus was tentatively named "walking iris virus 1" (WIV1). The complete genome of WIV1 is 2,858 nt in length with a single ORF encoding a viral replicase (RdRp). The highest level of amino acid sequence identity was 45% to Beta vulgaris mitovirus 1. In the viral replicase, a conserved protein domain for mitovirus RNA-dependent RNA polymerase and six highly conserved motifs were detected, consistent with other members of the family Mitoviridae. Phylogenetic inferences placed WIV1 among members of the genus Duamitovirus (family Mitoviridae) in a monophyletic clade with other plant mitoviruses. Sequence comparison and phylogenetic analysis support the classification of WIV1 as a new member of the genus Duamitovirus (family Mitoviridae).
通过高通量测序，从走茎鸢尾（Trimezia northiana (Schneev.) Ravenna）中获得了一种新的丝状病毒科成员的完整基因组序列。这是在单子叶植物中鉴定出的第一个假定的丝状病毒。新的丝状病毒暂时命名为“走茎鸢尾病毒 1”（WIV1）。WIV1 的完整基因组长 2858 个核苷酸，具有一个编码病毒复制酶（RdRp）的单一 ORF。与 Beta vulgaris mitovirus 1 的氨基酸序列相似性最高为 45%。在病毒复制酶中，检测到一个保守的丝状病毒 RNA 依赖性 RNA 聚合酶蛋白结构域和六个高度保守的基序，与丝状病毒科的其他成员一致。系统发育推断表明，WIV1 与 Duamitovirus 属的其他成员一起位于一个单系分支中，与其他植物丝状病毒一起。序列比较和系统发育分析支持将 WIV1 分类为 Duamitovirus 属（丝状病毒科）的一个新成员。
