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在干扰素合成缺陷的猿猴细胞中外源人β干扰素基因的转录及转录后调控

Transcriptional and posttranscriptional regulation of exogenous human beta interferon gene in simian cells defective in interferon synthesis.

作者信息

Mosca J D, Pitha P M

出版信息

Mol Cell Biol. 1986 Jun;6(6):2279-83. doi: 10.1128/mcb.6.6.2279-2283.1986.

DOI:10.1128/mcb.6.6.2279-2283.1986
PMID:3785197
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC367773/
Abstract

We determined that the defect in beta interferon induction in Vero cells is due to the absence of the simian beta interferon (IFN-beta) gene. Nevertheless, the human IFN-beta gene or a hybrid gene, in which the human IFN-beta promoter-regulatory region directs expression of the chloramphenicol acetyltransferase gene (pIFN-CAT), could be induced in transfected Vero cells, and these cells also regulated IFN-beta mRNA (but not pIFN-CAT mRNA) posttranscriptionally. These results indicate that the instability in the human IFN-beta gene is coded for by the coding or 3'-end region of IFN-beta mRNA and that the human IFN-beta gene is regulated in Vero and human cells in an identical manner.

摘要

我们确定，Vero细胞中β干扰素诱导缺陷是由于猿猴β干扰素（IFN-β）基因缺失所致。然而，人IFN-β基因或一种杂合基因（其中人IFN-β启动子调控区指导氯霉素乙酰转移酶基因（pIFN-CAT）的表达）在转染的Vero细胞中可被诱导，并且这些细胞在转录后也调控IFN-β mRNA（但不调控pIFN-CAT mRNA）。这些结果表明，人IFN-β基因的不稳定性由IFN-β mRNA的编码区或3'端区域编码，并且人IFN-β基因在Vero细胞和人细胞中的调控方式相同。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4a8/367773/fbd9262588e6/molcellb00090-0437-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4a8/367773/84deff60518a/molcellb00090-0436-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4a8/367773/28b42664632e/molcellb00090-0436-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4a8/367773/57a194789463/molcellb00090-0437-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4a8/367773/fbd9262588e6/molcellb00090-0437-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4a8/367773/84deff60518a/molcellb00090-0436-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4a8/367773/28b42664632e/molcellb00090-0436-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4a8/367773/57a194789463/molcellb00090-0437-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e4a8/367773/fbd9262588e6/molcellb00090-0437-b.jpg

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Regulated expression of human interferon beta 1 gene after transduction into cultured mouse and rabbit cells.转导至培养的小鼠和兔细胞后人类干扰素β1基因的调控表达。
Proc Natl Acad Sci U S A. 1982 Sep;79(17):5166-70. doi: 10.1073/pnas.79.17.5166.
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Regulated expression of an extrachromosomal human beta-interferon gene in mouse cells.
严重急性呼吸综合征冠状病毒2型（SARS-CoV-2）非结构蛋白15（nsp15）通过破坏宿主抗病毒防御来增强病毒毒力。
Proc Natl Acad Sci U S A. 2025 Jun 17;122(24):e2426528122. doi: 10.1073/pnas.2426528122. Epub 2025 Jun 12.
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A Novel Toolkit of SARS-CoV-2 Sub-Genomic Replicons for Efficient Antiviral Screening.一种用于高效抗病毒筛选的新型严重急性呼吸综合征冠状病毒2亚基因组复制子工具包。
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Chikungunya Replication and Infection Is Dependent upon and Alters Cellular Hexosylceramide Levels in Vero Cells.基孔肯雅病毒的复制和感染依赖于并改变了非洲绿猴肾细胞中的神经酰胺水平。
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