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Differential and cell type specific expression of murine alpha-interferon genes is regulated on the transcriptional level.

作者信息

Bisat F, Raj N B, Pitha P M

机构信息

Oncology Center, Johns Hopkins University School of Medicine, Baltimore, MD 21205.

出版信息

Nucleic Acids Res. 1988 Jul 11;16(13):6067-83. doi: 10.1093/nar/16.13.6067.

DOI:10.1093/nar/16.13.6067
PMID:3399385
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC336848/
Abstract

In mouse cells induced with virus infection or dsRNA, the relative levels of alpha-4 interferon mRNA were higher than the levels of alpha-1 and alpha-6 mRNAs; the ratio between relative levels of alpha-4 and alpha-1 or alpha-6 mRNA was, however, dependent on the cell type. Recombinant plasmids, in which the expression of chloramphenicol acetyltransferase (CAT) gene was directed by the promoter regions of alpha-1, alpha-4 or alpha-6 interferon genes were constructed and their inducible expression was studied either in transient assay or in permanently transfected mouse cells. The highest levels of CAT activity and CAT mRNA were observed with alpha-4 CAT plasmid, while the expression of alpha-1 CAT was consistently higher than that coded by alpha-6 CAT plasmid; the ratio between CAT activities coded by alpha-4 CAT and alpha-1 CAT was dependent on cell type. However, in heterologous Vero cells, the transfected alpha-1 and alpha-4 genes were expressed constitutively, and the levels of mRNAs were comparable. These results show that the difference in the relative levels of individual alpha-1 and alpha-4 mRNAs reflects the transcriptional inducibility of the respective promoter regions.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87b6/336848/f73f8b82c42a/nar00156-0369-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87b6/336848/879b5a22f765/nar00156-0363-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87b6/336848/37620d7096f6/nar00156-0364-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87b6/336848/6dbea7049a37/nar00156-0365-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87b6/336848/88c2a93097bf/nar00156-0366-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87b6/336848/c47f0a9f3f56/nar00156-0368-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87b6/336848/f73f8b82c42a/nar00156-0369-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87b6/336848/879b5a22f765/nar00156-0363-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87b6/336848/37620d7096f6/nar00156-0364-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87b6/336848/6dbea7049a37/nar00156-0365-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87b6/336848/88c2a93097bf/nar00156-0366-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87b6/336848/c47f0a9f3f56/nar00156-0368-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87b6/336848/f73f8b82c42a/nar00156-0369-a.jpg

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本文引用的文献

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Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.在哺乳动物细胞中表达氯霉素乙酰转移酶的重组基因组。
Mol Cell Biol. 1982 Sep;2(9):1044-51. doi: 10.1128/mcb.2.9.1044-1051.1982.
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Regulated expression of human interferon beta 1 gene after transduction into cultured mouse and rabbit cells.转导至培养的小鼠和兔细胞后人类干扰素β1基因的调控表达。
Proc Natl Acad Sci U S A. 1982 Sep;79(17):5166-70. doi: 10.1073/pnas.79.17.5166.
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Regulated expression of an extrachromosomal human beta-interferon gene in mouse cells.
Biochem J. 1996 Aug 1;317 ( Pt 3)(Pt 3):697-706. doi: 10.1042/bj3170697.
4
Inhibition of human immunodeficiency virus type 1 replication by a Tat-activated, transduced interferon gene: targeted expression to human immunodeficiency virus type 1-infected cells.通过Tat激活的转导干扰素基因抑制1型人类免疫缺陷病毒复制：靶向表达至1型人类免疫缺陷病毒感染细胞
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Stimulation of interferon and cytokine gene expression by imiquimod and stimulation by Sendai virus utilize similar signal transduction pathways.咪喹莫特对干扰素和细胞因子基因表达的刺激以及仙台病毒的刺激利用了相似的信号转导途径。
Mol Cell Biol. 1995 Apr;15(4):2207-18. doi: 10.1128/MCB.15.4.2207.
6
Repression of the murine interferon alpha 11 gene: identification of negatively acting sequences.小鼠干扰素α11基因的抑制：负性作用序列的鉴定
Nucleic Acids Res. 1991 Aug 25;19(16):4497-502. doi: 10.1093/nar/19.16.4497.
7
Distinct activation of murine interferon-alpha promoter region by IRF-1/ISFG-2 and virus infection.IRF-1/ISFG-2和病毒感染对小鼠干扰素-α启动子区域的特异性激活。
Nucleic Acids Res. 1992 Jun 11;20(11):2877-84. doi: 10.1093/nar/20.11.2877.
人β-干扰素基因在小鼠细胞中的染色体外调控表达。
Proc Natl Acad Sci U S A. 1982 Aug;79(16):4897-901. doi: 10.1073/pnas.79.16.4897.
4
Chromosomal localization of human leukocyte, fibroblast, and immune interferon genes by means of in situ hybridization.通过原位杂交对人白细胞、成纤维细胞和免疫干扰素基因进行染色体定位。
Proc Natl Acad Sci U S A. 1982 Dec;79(24):7809-13. doi: 10.1073/pnas.79.24.7809.
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Mapping of murine interferon-alpha genes to chromosome 4.小鼠α干扰素基因定位于4号染色体
Gene. 1983 Dec;26(2-3):181-8. doi: 10.1016/0378-1119(83)90188-9.
6
Two levels of regulation of beta-interferon gene expression in human cells.人类细胞中β-干扰素基因表达的两级调控
Proc Natl Acad Sci U S A. 1983 Jul;80(13):3923-7. doi: 10.1073/pnas.80.13.3923.
7
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8
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Nucleic Acids Res. 1983 Apr 25;11(8):2447-64. doi: 10.1093/nar/11.8.2447.
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Eur J Biochem. 1983 Jun 15;133(2):399-404. doi: 10.1111/j.1432-1033.1983.tb07476.x.