Animal Breeding Biology and Molecular Genetics, Albrecht Daniel Thaer-Institute for Agricultural and Horticultural Sciences, Humboldt-Universität zu Berlin, Berlin, Germany.
Institute of Animal Breeding and Genetics, Justus-Liebig-Universität, Giessen, Germany.
Vet Res. 2023 Oct 18;54(1):95. doi: 10.1186/s13567-023-01227-0.
When resequencing animal genomes, some short reads cannot be mapped to the reference genome and are usually discarded. In this study, unmapped reads from 302 German Black Pied cattle were analyzed to identify potential pathogenic DNA. These unmapped reads were assembled and blasted against NCBI's database to identify bacterial and viral sequences. The results provided evidence for the presence of pathogens. We found sequences of Bovine parvovirus 3 and Mycoplasma species. These findings emphasize the information content of unmapped reads for gaining insight into bacterial and viral infections, which is important for veterinarians and epidemiologists.
当对动物基因组进行重测序时，一些短读段无法映射到参考基因组，通常会被丢弃。在这项研究中，分析了 302 头德国黑皮牛的未映射读段，以鉴定潜在的致病 DNA。这些未映射的读段被组装并与 NCBI 的数据库进行比对，以鉴定细菌和病毒序列。结果提供了病原体存在的证据。我们发现了牛细小病毒 3 和支原体属的序列。这些发现强调了未映射读段对于了解细菌和病毒感染的信息含量，这对兽医和流行病学家很重要。
