TECAL Research Group, Meat and Meat Products Research Institute (IPROCAR), Universidad de Extremadura (UEX), Cáceres 10003, Spain.
Animal Health Department, Universidad of Extremadura (UEX), Cáceres 10003, Spain.
J Agric Food Chem. 2023 Nov 1;71(43):16270-16285. doi: 10.1021/acs.jafc.3c04515. Epub 2023 Oct 19.
The gastrointestinal tract (GIT) is the target of assorted pathological conditions, and dietary components are known to affect its functionality and health. In previous studies, we observed that reducing sugars induced protein glycoxidation and impaired protein digestibility. To gain further insights into the pathophysiological effects of dietary sugars, rats were provided with a 30% (w/v) fructose water solution for 10 weeks. Upon slaughter, protein digestibility was assessed, and the entire GIT (digests and tissues) was analyzed for markers of oxidative stress and untargeted metabolomics. Additionally, the impact of sustained fructose intake on colonic microbiota was also evaluated. High fructose intake for 10 weeks decreased protein digestibility and promoted changes in the physiological digestion of proteins, enhancing intestinal digestion rather than stomach digestion. Moreover, at colonic stages, the oxidative stress was harmfully increased, and both the microbiota and the intraluminal colonic metabolome were modified.
胃肠道（GIT）是各种病理状况的靶标，已知膳食成分会影响其功能和健康。在之前的研究中，我们观察到，减少糖会诱导蛋白质糖基化和蛋白质消化率降低。为了更深入地了解膳食糖的病理生理影响，给大鼠提供了 30%（w/v）的果糖水溶液，为期 10 周。屠宰后，评估蛋白质消化率，并分析整个胃肠道（消化物和组织）的氧化应激和非靶向代谢组学标志物。此外，还评估了持续摄入果糖对结肠微生物组的影响。10 周的高果糖摄入降低了蛋白质消化率，并促进了蛋白质生理消化的变化，增强了肠道消化而不是胃消化。此外，在结肠阶段，氧化应激受到有害的增加，肠道微生物组和腔内结肠代谢组都发生了改变。
