sp. nov.，分离自南极洲联合冰川的土壤样本。

sp. nov., isolated from a soil sample from Union Glacier, Antarctica.

机构信息

Universidad Andres Bello, BioNanotechnology and Microbiology Laboratory, Center for Bioinformatics and Integrative Biology (CBIB), Facultad de Ciencias de la Vida, Av. República #330, Santiago, Chile.

出版信息

Int J Syst Evol Microbiol. 2023 Oct;73(10). doi: 10.1099/ijsem.0.006095.

DOI:10.1099/ijsem.0.006095

PMID:37861393

Abstract

A Gram-stain-positive, catalase-positive, non-motile bacteria, with a rod-coccus cycle (designated as EH-1B-1) was isolated from a soil sample from Union Glacier in Ellsworth Mountains, Antarctica. Strain EH-1B-1 had an optimal growth temperature of 28 °C and grew at pH 7-10. The major cellular fatty acids were anteiso-C, iso-C, C and anteiso-C. The G+C content based on the whole genome sequence was 63.1 mol%. Strain EH-1B-1 was most closely related to members of the genus , namely and . The strain grew on tryptic soy agar, Reasoner's 2A agar, lysogeny broth agar and nutrient agar. The average nucleotide identity and digital DNA-DNA hybridization values between strain EH-1B-1 and its closest reference type strains ranged from 78 to 88 % and from 20.9 to 36.3 %, respectively. Based on phenotypic, chemotypic and genotypic evidence, it is proposed that strain EH-1B-1 represents a novel species of , for which the name sp. nov. is proposed, with strain EH-1B-1 (RGM 3386=LMG 32961) as the type strain.

摘要

一株革兰氏阳性、过氧化氢酶阳性、不运动的细菌，具有杆-球菌循环（命名为 EH-1B-1），从南极洲埃尔斯沃思山脉联合冰川的土壤样本中分离得到。EH-1B-1 株的最佳生长温度为 28°C，在 pH 值为 7-10 的条件下生长。主要细胞脂肪酸为 anteiso-C、iso-C、C 和 anteiso-C。基于全基因组序列的 G+C 含量为 63.1 mol%。EH-1B-1 株与属内的成员最为密切相关，即和。该菌株在胰蛋白酶大豆琼脂、雷纳 2A 琼脂、溶菌肉汤琼脂和营养琼脂上生长。EH-1B-1 株与其最接近的参考型菌株之间的平均核苷酸同一性和数字 DNA-DNA 杂交值分别为 78-88%和 20.9-36.3%。基于表型、化学型和基因型证据，建议 EH-1B-1 株代表一个新的属种，命名为 sp. nov.，EH-1B-1 株（RGM 3386=LMG 32961）作为模式菌株。