Faculty of Science, Division of Biological Science, Prince of Songkhla University, Hat Yai, Songkhla, Thailand.
Center of Antimicrobial Biomaterial Innovation-Southeast Asia, Prince of Songkhla University, Hat Yai, Songkhla, Thailand.
PLoS One. 2023 Oct 20;18(10):e0291505. doi: 10.1371/journal.pone.0291505. eCollection 2023.
The emergence of multidrug-resistant (MDR) pathogens poses a significant challenge for global public health systems, increasing hospital morbidity and mortality and prolonged hospitalization.
We evaluated the antimicrobial activity of a thermosensitive hydrogel containing bio-synthesized silver nanoparticles (bio-AgNPs) based on chitosan/poloxamer 407 using a leaf extract of Eucalyptus calmadulensis.
The thermosensitive hydrogel was prepared by a cold method after mixing the ingredients and left at 4°C overnight to ensure the complete solubilization of poloxamer 407. The stability of the hydrogel formulation was evaluated at room temperature for 3 months, and the absorption peak (420 nm) of the NPs remained unchanged. The hydrogel formulation demonstrated rapid gelation under physiological conditions, excellent water retention (85%), and broad-spectrum antimicrobial activity against MDR clinical isolates and ATCC strains. In this regard, minimum inhibitory concentration and minimum microbial concentration values of the bio-AgNPs ranged from 2-8 μg/mL to 8-128 μg/mL, respectively. Formulation at concentrations <64 μg/mL showed no cytotoxic effect on human-derived macrophages (THP-1 cells) with no induction of inflammation.
The formulated hydrogel could be used in biomedical applications as it possesses a broad antimicrobial spectrum and anti-inflammatory properties without toxic effects on human cells.
多药耐药（MDR）病原体的出现对全球公共卫生系统构成了重大挑战，增加了医院的发病率和死亡率，并延长了住院时间。
我们评估了基于壳聚糖/泊洛沙姆 407 的生物合成银纳米粒子（bio-AgNPs）的热敏水凝胶的抗菌活性，该水凝胶使用了桉树 calmadulensis 的叶提取物。
热敏水凝胶通过混合成分后采用冷法制备，并在 4°C 下放置过夜，以确保泊洛沙姆 407 完全溶解。在室温下评估水凝胶配方 3 个月的稳定性，纳米粒子的吸收峰（420nm）保持不变。水凝胶配方在生理条件下迅速凝胶化，具有出色的保水能力（85%），并对 MDR 临床分离株和 ATCC 菌株具有广谱抗菌活性。在这方面，bio-AgNPs 的最小抑菌浓度和最小微生物浓度值分别为 2-8μg/mL 至 8-128μg/mL。浓度<64μg/mL 的配方对人源性巨噬细胞（THP-1 细胞）没有细胞毒性作用，也没有引发炎症。
由于该配方水凝胶具有广谱抗菌谱和抗炎特性，且对人体细胞没有毒性作用，因此可用于生物医学应用。
