Sekhar Kanaparedu P C, Zhang Xunhui, Geng Huimin, Yu Qun, Zhang Peiyu, Cui Jiwei
Key Laboratory of Colloid and Interface Chemistry of the Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan, Shandong 250100, China.
State Key Laboratory of Microbial Technology, Shandong University, Qingdao, Shandong 266237, China.
Biomacromolecules. 2023 Nov 13;24(11):5394-5402. doi: 10.1021/acs.biomac.3c00840. Epub 2023 Oct 23.
Intrinsic hemostasis is an innate body response to prevent bleeding based on the sol-gel transition of blood. However, it is often inadequate for exceptional situations, such as acute injury and coagulation disorders, which typically require immediate medical intervention. Herein, we report the preparation of an efficient hemostatic powder, composed of tannic acid (TA), poly(ethylene glycol) (PEG), and poly(d,l-lactide--glycolide)--poly(ethylene glycol)--poly(d,l-lactide--glycolide) triblock copolymer (TB), for biomimetic hemostasis at the bleeding sites. TA has a high affinity for biomolecules and cells and can form coacervates with PEG driven by hydrogen bonding. TB enhances the mechanical strength and provides thermoresponsiveness. The hemostatic powder can rapidly transit into a physical and biodegradable seal on wet substrates under physiological conditions, demonstrating its promise for the generation of instant artificial clots. Importantly, this process is independent of the innate blood clotting process, which could benefit those with blood clotting disorders. This biomimetic hemostatic powder is an adaptive topical sealing agent for noncompressible and irregular wounds, which is promising for biomedical applications.
内源性止血是机体基于血液的溶胶-凝胶转变来预防出血的一种固有反应。然而，对于诸如急性损伤和凝血障碍等特殊情况，内源性止血往往并不充分，这些情况通常需要立即进行医学干预。在此，我们报告了一种高效止血粉的制备，该止血粉由单宁酸（TA）、聚乙二醇（PEG）和聚（d，l-丙交酯-乙交酯）-聚（乙二醇）-聚（d，l-丙交酯-乙交酯）三嵌段共聚物（TB）组成，用于在出血部位进行仿生止血。TA对生物分子和细胞具有高亲和力，并且可以在氢键驱动下与PEG形成凝聚层。TB增强了机械强度并提供热响应性。该止血粉在生理条件下可在湿底物上迅速转变为物理性且可生物降解的密封物，证明了其在生成即时人工血凝块方面的前景。重要的是，这一过程独立于内源性凝血过程，这对患有凝血障碍的人可能有益。这种仿生止血粉是一种适用于不可压缩和不规则伤口的局部密封剂，在生物医学应用方面具有广阔前景。
