College of Ocean Food and Biological Engineering, Jimei University, Xiamen, 361021, China.
World J Microbiol Biotechnol. 2023 Oct 25;39(12):356. doi: 10.1007/s11274-023-03810-1.
Postharvest anthracnose of mango fruit caused by Colletotrichum gloeosporioides is a devastating fungal disease, which causes tremendous quality deterioration and economic losses. Hinokitiol, an environmentally friendly natural compound, is effective in controlling a variety of postharvest fungal diseases. However, there is still a lack of research on the inhibitory effect of hinokitiol on C. gloeosporioides and its possible modes of action. In the present study, the activity of hinokitiol against C. gloeosporioides and its potential mechanisms involved have been investigated. We found that hinokitiol treatment could effectively inhibit the virulence of C. gloeosporioides to harvested mango fruit. After treatment with 8 mg/L hinokitiol, the mycelial growth of C. gloeosporioides was completely inhibited. When the concentration of hinokitiol reached 9 mg/L, the spore germination rate of C. gloeosporioides decreased to 2.43% after 9 h of cultivation. The inhibitory effect is mainly due to the attenuation in cell viability, and impairment in plasma membrane followed by leakage of cytoplasmic contents such as nucleic acids, proteins, and soluble carbohydrates, which ultimately leads to the destruction of cell structure. Furthermore, hinokitiol suppressed the expression of pathogenicity-related genes, leading to reduced infection activity. Collectively, these results suggest that hinokitiol may be an excellent bio-fungicides for the management of mango anthracnose.
采后芒果炭疽病是由胶孢炭疽菌引起的破坏性真菌病害，会导致严重的品质恶化和经济损失。桧木醇是一种环保的天然化合物，对控制多种采后真菌病害有效。然而，桧木醇对胶孢炭疽菌的抑制作用及其可能的作用方式仍缺乏研究。本研究探讨了桧木醇对胶孢炭疽菌的活性及其潜在机制。我们发现桧木醇处理能有效抑制胶孢炭疽菌对采后芒果果实的致病力。8mg/L 桧木醇处理完全抑制胶孢炭疽菌的菌丝生长，当桧木醇浓度达到 9mg/L 时，培养 9 小时后胶孢炭疽菌的孢子萌发率下降至 2.43%。抑制作用主要归因于细胞活力减弱，质膜受损，导致核酸、蛋白质和可溶性碳水化合物等细胞质内容物泄漏，最终破坏细胞结构。此外，桧木醇抑制了致病相关基因的表达，导致感染活性降低。综上所述，桧木醇可能是防治芒果炭疽病的优良生物杀菌剂。
