细胞学和转录组学分析揭示花生网斑病抗性的机制。

Cytological and transcriptomic analysis to unveil the mechanism of web blotch resistance in Peanut.

机构信息

The Shennong Laboratory, Institute of Crop Molecular Breeding, Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan Provincial Key Laboratory for Oil Crop Improvement, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, 450002, China.

College of Agronomy, Henan Agricultural University, Zhengzhou, Henan, 450046, China.

出版信息

BMC Plant Biol. 2023 Oct 26;23(1):518. doi: 10.1186/s12870-023-04545-9.

DOI:10.1186/s12870-023-04545-9

PMID:37884908

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10601179/

Abstract

BACKGROUND

Peanut is an important oil crop worldwide. Peanut web blotch is a fungal disease that often occurs at the same time as other leaf spot diseases, resulting in substantial leaf drop, which seriously affects the peanut yield and quality. However, the molecular mechanism underlying peanut resistance to web blotch is unknown.

RESULTS

The cytological examination revealed no differences in the conidium germination rate between the web blotch-resistant variety ZH and the web blotch-susceptible variety PI at 12-48 hpi. The appressorium formation rate was significantly higher for PI than for ZH at 24 hpi. The papilla formation rate at 36 hpi and the hypersensitive response rate at 60 and 84 hpi were significantly higher for ZH than for PI. We also compared the transcriptional profiles of web blotch-infected ZH and PI plants at 0, 12, 24, 36, 48, 60, and 84 hpi using an RNA-seq technique. There were more differentially expressed genes (DEGs) in ZH and PI at 12, 36, 60, and 84 hpi than at 24 and 48 hpi. Moreover, there were more DEGs in PI than in ZH at each time-point. The analysis of metabolic pathways indicated that pantothenate and CoA biosynthesis; monobactam biosynthesis; cutin, suberine and wax biosynthesis; and ether lipid metabolism are specific to the active defense of ZH against YY187, whereas porphyrin metabolism as well as taurine and hypotaurine metabolism are pathways specifically involved in the passive defense of ZH against YY187. In the protein-protein interaction (PPI) network, most of the interacting proteins were serine acetyltransferases and cysteine synthases, which are involved in the cysteine synthesis pathway. The qRT-PCR data confirmed the reliability of the transcriptome analysis.

CONCLUSION

On the basis of the PPI network for the significantly enriched genes in the pathways which were specifically enriched at different time points in ZH, we hypothesize that serine acetyltransferases and cysteine synthases are crucial for the cysteine-related resistance of peanut to web blotch. The study results provide reference material for future research on the mechanism mediating peanut web blotch resistance.

摘要

背景

花生是世界范围内重要的油料作物。花生网斑病是一种真菌病害，常与其他叶斑病同时发生，导致大量叶片脱落，严重影响花生的产量和品质。然而，花生抗网斑病的分子机制尚不清楚。

结果

细胞学检查发现，抗网斑病品种 ZH 和感网斑病品种 PI 在 12-48 hpi 时，分生孢子萌发率无差异。PI 的附着胞形成率在 24 hpi 时显著高于 ZH。在 36 hpi 时，PI 的乳突形成率和在 60 和 84 hpi 时的过敏性反应率显著高于 ZH。我们还使用 RNA-seq 技术比较了感染网斑病的 ZH 和 PI 植株在 0、12、24、36、48、60 和 84 hpi 时的转录谱。在 12、36、60 和 84 hpi 时，ZH 和 PI 中的差异表达基因（DEGs）多于 24 和 48 hpi。此外，在每个时间点，PI 中的 DEGs 多于 ZH。代谢途径分析表明，泛酸和辅酶 A 生物合成；单环β-内酰胺生物合成；角质、栓皮和蜡生物合成；醚脂代谢是 ZH 对 YY187 主动防御所特有的，而卟啉代谢以及牛磺酸和次牛磺酸代谢是 ZH 对 YY187 被动防御所特有的途径。在蛋白质-蛋白质相互作用（PPI）网络中，大多数相互作用的蛋白质是丝氨酸乙酰转移酶和半胱氨酸合酶，它们参与半胱氨酸合成途径。qRT-PCR 数据证实了转录组分析的可靠性。