Wang Tao, Luo Yuheng, Kong Xiangfeng, Yu Bing, Zheng Ping, Huang Zhiqing, Mao Xiangbing, Yu Jie, Luo Junqiu, Yan Hui, He Jun
Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, China.
Key Laboratory of Animal Disease-Resistant Nutrition, Chengdu 611130, China.
Microorganisms. 2023 Sep 22;11(10):2370. doi: 10.3390/microorganisms11102370.
Comprehensive studies on the effects of genetics and fiber diets on antibiotic resistance genes (ARGs) remain scarce. In this study, we analyzed the profiles of ARGs in colonic contents and fecal samples of Taoyuan, Duroc, and Xiangcun pigs (n = 10) fed at different fiber levels. Through macrogenomic analysis, we identified a total of 850 unique types of ARGs and classified them into 111 drug resistance classes. The abundance of partially drug-resistant ARGs was higher in the colonic contents of local pig breeds under a large-scale farming model. ARGs were found to be widely distributed among a variety of bacteria, predominantly in the phyla , , and . Fiber diets reduce the abundance of ARGs in colonic contents and feces, and mobile genetic elements (MGEs) and short-chain fatty acids (SCFAs) are important drivers in mediating the effect of fiber diets on the abundance of ARGs. In vitro fermentation experiments confirmed that butyric acid significantly reduced the abundance of ARGs. In summary, the results of this study enhanced our understanding of the distribution and composition of ARGs in the colon of different breeds of pigs and revealed that a fiber diet can reduce ARGs in feces through its Butyric acid, providing reference data for environmental safety.
关于遗传学和纤维饮食对抗生素抗性基因（ARGs）影响的综合研究仍然很少。在本研究中，我们分析了不同纤维水平饲养的桃源猪、杜洛克猪和香村猪（n = 10）结肠内容物和粪便样本中ARGs的谱图。通过宏基因组分析，我们共鉴定出850种独特类型的ARGs，并将它们分为111个耐药类别。在大规模养殖模式下，本地猪种结肠内容物中部分耐药ARGs的丰度较高。发现ARGs广泛分布于多种细菌中，主要存在于门、门和门中。纤维饮食可降低结肠内容物和粪便中ARGs的丰度，移动遗传元件（MGEs）和短链脂肪酸（SCFAs）是介导纤维饮食对ARGs丰度影响的重要驱动因素。体外发酵实验证实丁酸可显著降低ARGs的丰度。总之，本研究结果加深了我们对不同品种猪结肠中ARGs分布和组成的理解，并揭示了纤维饮食可通过丁酸降低粪便中的ARGs，为环境安全提供了参考数据。
