大肠杆菌中染色体β-内酰胺酶的体内调控
In vivo regulation of chromosomal beta-lactamase in Escherichia coli.
作者信息
Jaurin B, Normark S
出版信息
J Bacteriol. 1979 Jun;138(3):896-902. doi: 10.1128/jb.138.3.896-902.1979.
Abstract
Chromosomal beta-lactamase, a periplasmic enzyme of Escherichia coli, was studied with respect to its regulation in vivo. Both the activity and the amount of beta-lactamase increased with growth rate. During a nutritional shift-down, chromosomal beta-lactamase activity followed stable ribonucleic acid accumulation. After a nutritional shift-up the differential rate of beta-lactamase synthesis did not increase immediately (like stable ribonucleic acid), but did increase after a lag period of 30 min. To determine whether beta-lactamase was under stringent control, strains carrying a temperature-sensitive valyl-transfer ribonucleic acid synthetase and differing only in the allelic state of the relA gene were shifted from a permissive to a semipermissive temperature. No influence by the relA gene product was found on beta-lactamase synthesis. The regulation of this periplasmic enzyme is discussed in relation to that of some components of the translational apparatus.
摘要
对大肠杆菌的一种周质酶——染色体β-内酰胺酶的体内调控进行了研究。β-内酰胺酶的活性和含量均随生长速率增加。在营养条件向下转变期间，染色体β-内酰胺酶活性与稳定核糖核酸积累情况一致。营养条件向上转变后，β-内酰胺酶合成的差异速率并未立即增加（与稳定核糖核酸情况不同），而是在30分钟的延迟期后才增加。为了确定β-内酰胺酶是否受到严谨控制，将携带温度敏感型缬氨酰转移核糖核酸合成酶且仅relA基因等位状态不同的菌株从允许温度转移至半允许温度。未发现relA基因产物对β-内酰胺酶合成有影响。结合翻译装置的某些组分对这种周质酶的调控进行了讨论。
相似文献
1
In vivo regulation of chromosomal beta-lactamase in Escherichia coli.大肠杆菌中染色体β-内酰胺酶的体内调控
J Bacteriol. 1979 Jun;138(3):896-902. doi: 10.1128/jb.138.3.896-902.1979.
2
Stringent regulation of the synthesis of a transfer ribonucleic acid biosynthetic enzyme: transfer ribonucleic acid(m5U)methyltransferase from Escherichia coli.对一种转移核糖核酸生物合成酶合成的严格调控：来自大肠杆菌的转移核糖核酸（m5U）甲基转移酶
J Bacteriol. 1977 May;130(2):635-41. doi: 10.1128/jb.130.2.635-641.1977.
3
Regulation of the biosynthesis of aminoacyl-transfer ribonucleic acid synthetases and of transfer ribonucleic acid in Escherichia coli. V. Mutants with increased levels of valyl-transfer ribonucleic acid synthetase.大肠杆菌中氨酰基转移核糖核酸合成酶生物合成及转移核糖核酸的调控。V. 缬氨酰转移核糖核酸合成酶水平升高的突变体。
J Bacteriol. 1979 Jul;139(1):165-75. doi: 10.1128/jb.139.1.165-175.1979.
4
Cloning and expression of a cloxacillin-hydrolyzing enzyme and a cephalosporinase from Aeromonas sobria AER 14M in Escherichia coli: requirement for an E. coli chromosomal mutation for efficient expression of the class D enzyme.嗜水气单胞菌AER 14M中一种氯唑西林水解酶和一种头孢菌素酶在大肠杆菌中的克隆与表达：D类酶高效表达对大肠杆菌染色体突变的需求
Antimicrob Agents Chemother. 1994 Sep;38(9):2078-85. doi: 10.1128/AAC.38.9.2078.
5
Increased expression levels of chromosomal AmpC β-lactamase in clinical Escherichia coli isolates and their effect on susceptibility to extended-spectrum cephalosporins.临床大肠埃希菌分离株中染色体AmpCβ-内酰胺酶表达水平的增加及其对超广谱头孢菌素敏感性的影响。
Microb Drug Resist. 2015 Feb;21(1):7-16. doi: 10.1089/mdr.2014.0108. Epub 2014 Sep 4.
6
Secretion of beta-lactamase by Escherichia coli in vivo and in vitro: effect of cerulenin.大肠杆菌在体内和体外分泌β-内酰胺酶：浅蓝菌素的作用。
Antonie Van Leeuwenhoek. 1982;48(4):353-64. doi: 10.1007/BF00418288.
7
Reinitiation of deoxyribonucleic acid synthesis by deoxyribonucleic acid initiation mutants of Escherichia coli: role of ribonucleic acid synthesis, protein synthesis, and cell division.大肠杆菌脱氧核糖核酸起始突变体引发脱氧核糖核酸合成的重新启动：核糖核酸合成、蛋白质合成及细胞分裂的作用
J Bacteriol. 1975 Jan;121(1):219-26. doi: 10.1128/jb.121.1.219-226.1975.
8
[Beta-lactamase synthesis and excretion in a non-leaky wild strain and a leaky mutant of Escherichia coli K-12].[β-内酰胺酶在大肠杆菌K-12非渗漏野生菌株和渗漏突变体中的合成与排泄]
C R Seances Acad Sci III. 1983 Jan 17;296(2):119-22.
9
Biochemical and genetic characterization of a mutant of Escherichia coli with a temperature-sensitive valyl ribonucleic acid synthetase.具有温度敏感性缬氨酰核糖核酸合成酶的大肠杆菌突变体的生化与遗传特性
J Bacteriol. 1966 Oct;92(4):1076-82. doi: 10.1128/jb.92.4.1076-1082.1966.
10
Translational repression of TEM beta-lactamase synthesis as a response of Escherichia coli to heat shock.
Mol Microbiol. 1989 Aug;3(8):1131-40. doi: 10.1111/j.1365-2958.1989.tb00263.x.
引用本文的文献
1
Induction of AmpC-Mediated β-Lactam Resistance Requires a Single Lytic Transglycosylase in Agrobacterium tumefaciens.诱导 AmpC 介导的β-内酰胺耐药性仅需在根瘤农杆菌中单一的溶菌转糖基酶。
Appl Environ Microbiol. 2022 Jun 28;88(12):e0033322. doi: 10.1128/aem.00333-22. Epub 2022 May 31.
2
Physicochemical Factors That Favor Conjugation of an Antibiotic Resistant Plasmid in Non-growing Bacterial Cultures in the Absence and Presence of Antibiotics.在有无抗生素存在的情况下，有利于非生长细菌培养物中抗生素抗性质粒接合的物理化学因素。
Front Microbiol. 2018 Sep 11;9:2122. doi: 10.3389/fmicb.2018.02122. eCollection 2018.
3
Beta-lactam antibiotics: from antibiosis to resistance and bacteriology.β-内酰胺类抗生素：从抗菌作用到耐药性和细菌学。
APMIS. 2010 Jan;118(1):1-36. doi: 10.1111/j.1600-0463.2009.02563.x.
4
ampC cephalosporinase of Escherichia coli K-12 has a different evolutionary origin from that of beta-lactamases of the penicillinase type.大肠杆菌K-12的AmpC头孢菌素酶与青霉素酶型β-内酰胺酶有着不同的进化起源。
Proc Natl Acad Sci U S A. 1981 Aug;78(8):4897-901. doi: 10.1073/pnas.78.8.4897.
5
Identification of a novel ampC beta-lactamase promoter in a clinical isolate of Escherichia coli.在一株临床分离的大肠杆菌中鉴定出一种新型AmpC β-内酰胺酶启动子。
EMBO J. 1982;1(11):1411-6. doi: 10.1002/j.1460-2075.1982.tb01331.x.
6
ampC beta-lactamase hyperproduction in Escherichia coli: natural ampicillin resistance generated by horizontal chromosomal DNA transfer from Shigella.大肠杆菌中AmpCβ-内酰胺酶的高产：由志贺氏菌水平染色体DNA转移产生的天然氨苄西林耐药性
Proc Natl Acad Sci U S A. 1983 Dec;80(24):7556-60. doi: 10.1073/pnas.80.24.7556.
7
Sequence elements determining ampC promoter strength in E. coli.决定大肠杆菌中AmpC启动子强度的序列元件。
EMBO J. 1982;1(7):875-81. doi: 10.1002/j.1460-2075.1982.tb01263.x.
8
Molecular cloning, correlation of genetic and restriction maps, and determination of the direction of transcription of gnd of Escherichia coli.大肠杆菌gnd基因的分子克隆、遗传图谱与限制酶切图谱的关联以及转录方向的确定。
J Bacteriol. 1980 Aug;143(2):731-41. doi: 10.1128/jb.143.2.731-741.1980.
9
Different control circuits for growth rate-dependent regulation of 6-phosphogluconate dehydrogenase and protein components of the translational machinery in Escherichia coli.大肠杆菌中6-磷酸葡萄糖酸脱氢酶和翻译机制蛋白质成分的生长速率依赖性调控的不同控制电路。
J Bacteriol. 1982 Nov;152(2):584-94. doi: 10.1128/jb.152.2.584-594.1982.
10
Initiation of translation makes attenuation of ampC in E. coli dependent on growth rate.翻译起始使得大肠杆菌中AmpC的弱化依赖于生长速率。
Mol Gen Genet. 1985;198(3):411-5. doi: 10.1007/BF00332931.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Studies on lysogenesis. I. The mode of phage liberation by lysogenic Escherichia coli.溶源性研究。I. 溶源性大肠杆菌释放噬菌体的方式。
J Bacteriol. 1951 Sep;62(3):293-300. doi: 10.1128/jb.62.3.293-300.1951.
3
Acetylornithinase of Escherichia coli: partial purification and some properties.大肠杆菌的乙酰鸟氨酸酶：部分纯化及某些性质
J Biol Chem. 1956 Jan;218(1):97-106.
4
Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies.在含有抗体的琼脂糖凝胶中通过电泳对蛋白质进行定量估计。
Anal Biochem. 1966 Apr;15(1):45-52. doi: 10.1016/0003-2697(66)90246-6.
5
Resistance of Escherichia coli to penicillins. VI. Purification and characterization of the chromosomally mediated penicillinase present in ampA-containing strains.大肠杆菌对青霉素的耐药性。VI. 含ampA菌株中染色体介导的青霉素酶的纯化及特性分析。
J Bacteriol. 1970 Jan;101(1):218-31. doi: 10.1128/jb.101.1.218-231.1970.
6
On the regulation of guanosine tetraphosphate levels in stringent and relaxed strains of Escherichia coli.关于大肠杆菌严谨型和松弛型菌株中鸟苷四磷酸水平的调控
J Biol Chem. 1971 Jul 25;246(14):4381-5.
7
Automated method for determination of penicillins, cephalosporins, and penicillinases.青霉素、头孢菌素和青霉素酶的自动化测定方法。
Antimicrob Agents Chemother. 1972 Feb;1(2):100-6. doi: 10.1128/AAC.1.2.100.
8
Stringent control of ribosomal protein gene expression in Escherichia coli.大肠杆菌中核糖体蛋白基因表达的严格控制。
Proc Natl Acad Sci U S A. 1974 Oct;71(10):3819-23. doi: 10.1073/pnas.71.10.3819.
9
Culture medium for enterobacteria.用于肠道细菌的培养基。
J Bacteriol. 1974 Sep;119(3):736-47. doi: 10.1128/jb.119.3.736-747.1974.
10
Metabolic regulation of the arginyl and valyl transfer ribonucleic acid synthetases in bacteria.细菌中精氨酰和缬氨酰转移核糖核酸合成酶的代谢调控
J Biol Chem. 1974 Feb 25;249(4):1044-53.
Zotero 插件