大肠杆菌K-12中的新型吖啶黄素抗性基因acrC和acrD。
Novel acriflavin resistance genes, acrC and acrD, in Escherichia coli K-12.
作者信息
Nakamura H
出版信息
J Bacteriol. 1979 Jul;139(1):8-12. doi: 10.1128/jb.139.1.8-12.1979.
Acriflavine-resistant mutants were isolated from an acriflavine-sensitive (acrA) strain of Escherichia coli K-12 and then tested for temperature sensitivity of cell division. Genetic analysis characterized two new genetic loci, acrC and acrD. The former was mapped between tonA and proA, and the latter between the origin of genetic transfer of HfrH and serB. acrC and acrD mutants could divide but did not initiate a new round of deoxyribonucleic acid (DNA) replication at 43 degrees C. DNA synthesis of the acrC mutant cells ceased after a period of time following temperature shift-up, and thereafter DNA degradation occurred. However, cell mass continued to increase for a long time at the nonpermissive temperature. On the other hand, DNA synthesis of the acrD mutant cells ceased soon after the shift-up, and the cell mass did not appreciably increase during the prolonged incubation.
从大肠杆菌K-12的吖啶黄素敏感（acrA）菌株中分离出吖啶黄素抗性突变体，然后检测其细胞分裂的温度敏感性。遗传分析鉴定出两个新的基因座，acrC和acrD。前者定位于tonA和proA之间，后者定位于HfrH的遗传转移起点和serB之间。acrC和acrD突变体能够分裂，但在43℃时不能启动新一轮的脱氧核糖核酸（DNA）复制。温度升高一段时间后，acrC突变体细胞的DNA合成停止，随后发生DNA降解。然而，在非允许温度下，细胞质量持续增加很长时间。另一方面，acrD突变体细胞的DNA合成在温度升高后很快停止，并且在长时间孵育期间细胞质量没有明显增加。
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