Fera Science Limited, York Biotech Campus, York YO41 1LZ, UK.
Viruses. 2023 Oct 11;15(10):2076. doi: 10.3390/v15102076.
Tomato brown rugose fruit virus (ToBRFV) is a contact-transmitted tobamovirus affecting many tomato growing regions of the world. This study investigated the effects of different glasshouse surfaces on the survival of the virus; the efficacy of different disinfectants; and heat treatment against ToBRFV (surfaces included steel, aluminium, hard plastic, polythene, glass and concrete). A bioassay followed by ELISA was used to check virus viability. ToBRFV survived for at least 7 days on all surfaces tested and on some for at least 6 months. The virus survived for over two hours on hands and gloves. Hand washing was shown to be unreliable for the removal of the virus. Glutaraldehyde and quaternary ammonium compound disinfectants were effective at one hour on all surfaces. Some other disinfectants were effective at one hour of contact time, on all surfaces except concrete. Sodium hypochlorite was partially effective against ToBRFV, even on concrete. A 5 min soak of plastic trays in water at 90 °C was effective at denaturing ToBRFV; however, 5 min at 70 °C was not. Heating infected sap showed the thermal inactivation point to be 90 °C, confirming the hot water treatment results and showing that deactivation was due to the heat treatment and not a washing effect of the water.
番茄褐色皱果病毒（ToBRFV）是一种接触传播的烟草花叶病毒，影响着世界上许多番茄种植地区。本研究调查了不同温室表面对病毒存活的影响；不同消毒剂的功效；以及针对 ToBRFV 的热处理（表面包括钢、铝、硬塑料、聚乙烯、玻璃和混凝土）。通过生物测定和 ELISA 检测来检查病毒的生存能力。ToBRFV 在所有测试的表面上至少存活 7 天，在某些表面上至少存活 6 个月。病毒在手上和手套上至少能存活两个多小时。洗手在去除病毒方面并不可靠。戊二醛和季铵化合物消毒剂在所有表面上一小时内都有效。其他一些消毒剂在一小时的接触时间内对所有表面（除混凝土外）都有效。次氯酸钠对 ToBRFV 有一定的效果，即使在混凝土上也是如此。将塑料托盘在 90°C 的水中浸泡 5 分钟可有效使 ToBRFV 变性；然而，70°C 浸泡 5 分钟则不行。加热受感染的汁液表明其热失活点为 90°C，证实了热水处理结果，并表明失活是由于热处理而不是水的洗涤作用。
