Locarnini S A, Coulepis A G, Stratton A M, Kaldor J, Gust I D
J Clin Microbiol. 1979 Apr;9(4):459-65. doi: 10.1128/jcm.9.4.459-465.1979.
A solid-phase enzyme linked immunosorbent assay was developed for the detection of immunoglobulin M antibody to hepatitis A virus. The system was capable of detecting hepatitis A-specific immunoglobulin M in a single dilution of serum and appears to be a reliable and rapid means of establishing a diagnosis of hepatitis A infection. Specific immunoglobulin M was only detected in patients with serologically confirmed hepatitis A and not in patients with other forms of hepatitis, chronic liver disease, or autoimmune disease. In patients with hepatitis A, specific immunoglobulin M was usually detectable for 6 weeks after the onset of dark urine, and the longest period for which it was present in any patient was 115 days. This enzyme-linked immunosorbent assay is rapid, simple to perform, and does not require complicated equipment. Provided adequate supplies of purified reagents can be obtained, this enzyme-linked immunosorbent assay procedure is likely to simplify hepatitis A serology, because the same antibody-coated plates can be utilized to detect hepatitis A virus, anti-hepatitis A virus, and hepatitis A-specific immunoglobulin M.
开发了一种固相酶联免疫吸附测定法用于检测甲型肝炎病毒免疫球蛋白M抗体。该系统能够在血清单次稀释中检测甲型肝炎特异性免疫球蛋白M，似乎是建立甲型肝炎感染诊断的可靠且快速的方法。特异性免疫球蛋白M仅在血清学确诊的甲型肝炎患者中检测到，而在其他形式的肝炎、慢性肝病或自身免疫性疾病患者中未检测到。在甲型肝炎患者中，特异性免疫球蛋白M通常在出现深色尿后6周内可检测到，任何患者中其存在的最长时间为115天。这种酶联免疫吸附测定法快速、操作简单，且不需要复杂的设备。如果能够获得足够的纯化试剂供应，这种酶联免疫吸附测定法程序可能会简化甲型肝炎血清学检测，因为相同的抗体包被板可用于检测甲型肝炎病毒、抗甲型肝炎病毒和甲型肝炎特异性免疫球蛋白M。
