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中间偃麦草及其潜在祖先物种标准核型的构建

The Construction of a Standard Karyotype of Intermediate Wheatgrass and Its Potential Progenitor Species.

作者信息

Wang Lin, Liang Shuang, Qi Fei, Bao Yinguang, Wang Richard R-C, Li Xingfeng

机构信息

State Key Laboratory of Wheat Improvement, Shandong Agricultural University, Tai'an 271018, China.

Tai'an Subcenter of National Wheat Improvement Center, Agronomy College, Shandong Agricultural University, Tai'an 271018, China.

出版信息

Plants (Basel). 2025 Jan 12;14(2):196. doi: 10.3390/plants14020196.

DOI:10.3390/plants14020196
PMID:39861549
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11769444/
Abstract

The genome composition of intermediate wheatgrass (IWG; (Host) Barkworth and D.R. Dewey; 2n = 6x = 42) is complex and remains to be a subject of ongoing investigation. This study employed fluorescence in situ hybridization (FISH) to analyze the karyotype of and its related species. With the St-80 probe derived from and the pDb12H probe from , FISH analysis classified the chromosomes of as . FISH karyotype was established using pSc119.2-1, (GAA), AFA-3, AFA-4, pAs1-1, pAs1-3, pAs1-4, and pAs1-6 as a combined multiplex oligonucleotide probe. MATO software was used to analyze chromosome length, arm ratio, and karyotype structure. The karyotype formula of is K(2n) = 6X = 42 = 36m + 6sm, and that of is K(2n) = 4X = 28 = 22m + 6sm. The karyotype formula of and is K(2n) = 2X = 14 = 12m + 2sm, of is K(2n) = 2X = 14 = 2M + 12m, and of is K(2n) = 2X = 14 = 12m + 2sm. Based on the results of FISH, standard karyotypes of and its potential progenitor species were constructed. These standard karyotypes revealed that there was evolutionary parallelism between genome and karyotype, but due to the complexity of evolution, the FISH signal of was abundant and asymmetrical.

摘要

中间偃麦草（IWG；(Host) Barkworth和D.R. Dewey；2n = 6x = 42）的基因组组成复杂，仍是一个正在进行研究的课题。本研究采用荧光原位杂交（FISH）技术分析了中间偃麦草及其相关物种的核型。利用源自拟鹅观草的St - 80探针和来自大麦的pDb12H探针，FISH分析将中间偃麦草的染色体分类为……。使用pSc119.2 - 1、(GAA)、AFA - 3、AFA - 4、pAs1 - 1、pAs1 - 3、pAs1 - 4和pAs1 - 6作为复合多重寡核苷酸探针构建了FISH核型。使用MATO软件分析染色体长度、臂比和核型结构。中间偃麦草的核型公式为K(2n) = 6X = 42 = 36m + 6sm，某物种的核型公式为K(2n) = 4X = 28 = 22m + 6sm。某物种和某物种的核型公式为K(2n) = 2X = 14 = 12m + 2sm，某物种的核型公式为K(2n) = 2X = 14 = 2M + 12m，某物种的核型公式为K(2n) = 2X = 14 = 12m + 2sm。基于FISH结果，构建了中间偃麦草及其潜在祖先物种的标准核型。这些标准核型表明基因组和核型之间存在进化平行性，但由于进化的复杂性，中间偃麦草的FISH信号丰富且不对称。 （注：原文中部分物种名未完整给出，翻译时用“……”表示）

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d05/11769444/6035ae25776d/plants-14-00196-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d05/11769444/ec4f61aefd10/plants-14-00196-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d05/11769444/cb791e3b6fbd/plants-14-00196-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d05/11769444/6b44e9e823d3/plants-14-00196-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d05/11769444/6035ae25776d/plants-14-00196-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d05/11769444/ec4f61aefd10/plants-14-00196-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d05/11769444/cb791e3b6fbd/plants-14-00196-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d05/11769444/6b44e9e823d3/plants-14-00196-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d05/11769444/6035ae25776d/plants-14-00196-g004.jpg

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