Department of Chemistry, Amrit Campus, Tribhuvan University, 44613, Kathmandu, Nepal.
Research Center for Materials Nanoarchitectonics (MANA), National Institute for Materials Science (NIMS), 1-1 Namiki, 305-0044, Tsukuba, Ibaraki, Japan.
Chem Biodivers. 2023 Dec;20(12):e202301209. doi: 10.1002/cbdv.202301209. Epub 2023 Nov 14.
Genus Corydalis is a rich source of isoquinoline alkaloids reported to having potential bioactivities. Corydalis chaerophylla collected from Nepal at an altitude of 2400-4800 m was extracted using hexane, methanol and chloroform as solvents. The resulting hexane, methanol and chloroform extracts were subjected to LC-DAD-MS analysis to yield fifteen different alkaloids. To assess any potential pharmacological properties, antimicrobial activity against two Gram-positive, two Gram-negative bacterial strains and one fungal strain was assessed, revealing significant inhibitive action of the methanol and chloroform extracts. Of the extracts obtained using chloroform contained the highest content of phenolic compounds at 113 mg GAE/g, while the highest total flavonoid content was found for the hexane extract with a value of 46.45 mg QE/g. The chloroform extract also exhibited a considerable antioxidant activity at IC value, 261.5±3 μg/mL, for the DPPH assay. Conversely, the methanol extract exhibited the highest LC value for Brine Shrimp cytotoxicity at 196±3 μg/mL being least potential for the test. The methanol extract was found to be the most active against α-amylase inhibition with an IC of 51.52±2 μg/mL. In an in vivo acute oral toxicity study against mice, methanol and chloroform extracts presented harmful effects with 1000.36 mg/kg BW and 515 mg/kg BW for LD , respectively. By analyzing all the results of the solvents used, the chloroform extract was found to be the most active, a feature that will be used in future isolation procedures and other pharmacological tests.
紫堇属是异喹啉生物碱的丰富来源，据报道具有潜在的生物活性。从尼泊尔海拔 2400-4800 米处采集的紫堇属植物 chaerophylla 分别用正己烷、甲醇和氯仿作为溶剂进行提取。将得到的正己烷、甲醇和氯仿提取物进行 LC-DAD-MS 分析，得到十五种不同的生物碱。为了评估任何潜在的药理特性，对抗两种革兰氏阳性、两种革兰氏阴性细菌菌株和一种真菌菌株的抗菌活性进行了评估，结果表明甲醇和氯仿提取物具有显著的抑制作用。用氯仿提取的提取物含有最高含量的酚类化合物，为 113mgGAE/g，而正己烷提取物的总黄酮含量最高，为 46.45mgQE/g。氯仿提取物在 DPPH 测定中的 IC 值为 261.5±3μg/mL，表现出相当大的抗氧化活性。相反，甲醇提取物在盐水虾细胞毒性试验中的 LC 值最高，为 196±3μg/mL，对测试的潜在毒性最小。甲醇提取物对α-淀粉酶抑制的活性最高，IC 为 51.52±2μg/mL。在对小鼠进行的急性口服毒性研究中，甲醇和氯仿提取物的 LD 分别为 1000.36mg/kg BW 和 515mg/kg BW，呈现出有害影响。通过分析所有溶剂的结果，发现氯仿提取物最活跃，这一特性将用于未来的分离程序和其他药理测试。
