Shirvanizadeh Fatemeh, Eidi Akram, Hafezi Maryam, Eftekhari-Yazdi Poopak
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Department of Endocrinology and Female Infertility, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
JBRA Assist Reprod. 2023 Nov 14;27(4):682-8. doi: 10.5935/1518-0557.20230065.
Polycystic ovary syndrome (PCOS) is an endocrine disorder that seems to be pro-inflammatory at many levels, abdominal obesity (AO) is a prevalent pro-inflammatory phenotype in PCOS patients, and it seems to contribute to the initiation or worsening of inflammation in PCOS patients. In this study, we investigated the role of the AO phenotype in the occurrence of other obesity indicators (neck and arm) and augmentation of inflammation in the follicular fluid (FF) of PCOS patients.
40 patients under the age of 35 were divided into four groups: PCOS with AO, PCOS without AO, non-PCOS with AO, and non-PCOS without AO. The FF samples were collected from each patient. Clinical and anthropometric characteristics of the participants, as well as tumor necrosis factor-α (TNF-α) concentration in the FF samples, were quantitatively assessed using enzyme-linked immunosorbent assays. The number of retrieved cumulus-oocyte complexes (COC) and their quality were scored.
The PCOS+AO+ group had significantly increased neck circumference, compared to the other groups (p<0.001). The concentration of TNF-α was significantly higher in the PCOS+AO+ group than in the other groups (p<0.001). There were no significant differences in the number of retrieved COC per patient and the quality of oocytes between the groups (p>0.05).
Given the significant role of inflammation in the development of PCOS, managing AO in PCOS patients may aid in reducing inflammation and could potentially help in the design of customized treatment approaches.
多囊卵巢综合征（PCOS）是一种内分泌紊乱疾病，在多个层面似乎都具有促炎性质，腹部肥胖（AO）是PCOS患者中普遍存在的促炎表型，且似乎会促使PCOS患者炎症的发生或加重。在本研究中，我们调查了AO表型在PCOS患者其他肥胖指标（颈部和手臂）发生中的作用以及对卵泡液（FF）中炎症加剧的影响。
将40名35岁以下的患者分为四组：患有AO的PCOS患者、不患有AO的PCOS患者、患有AO的非PCOS患者和不患有AO的非PCOS患者。从每位患者采集FF样本。使用酶联免疫吸附测定法定量评估参与者的临床和人体测量特征，以及FF样本中肿瘤坏死因子-α（TNF-α）的浓度。对回收的卵丘-卵母细胞复合体（COC）数量及其质量进行评分。
与其他组相比，PCOS+AO+组的颈围显著增加（p<0.001）。PCOS+AO+组中TNF-α的浓度显著高于其他组（p<0.001）。各组间每位患者回收的COC数量和卵母细胞质量无显著差异（p>0.05）。
鉴于炎症在PCOS发展中的重要作用，控制PCOS患者的AO可能有助于减轻炎症，并可能有助于设计定制化的治疗方法。
