State Key Laboratory of Digital Medical Engineering, School of Biological Science and Medical Engineering, Southeast University, 210096, Nanjing, Jiangsu, China.
Institute of Biomaterials and Medical Devices, Southeast University, 215163, Suzhou, Jiangsu, China.
Nat Commun. 2023 Nov 14;14(1):7369. doi: 10.1038/s41467-023-43090-9.
Current techniques for visualizing and quantifying cellular forces have limitations in live cell imaging, throughput, and multi-scale analysis, which impede progress in cell force research and its practical applications. We developed a photonic crystal cellular force microscopy (PCCFM) to image vertical cell forces over a wide field of view (1.3 mm ⨯ 1.0 mm, a 10 ⨯ objective image) at high speed (about 20 frames per second) without references. The photonic crystal hydrogel substrate (PCS) converts micro-nano deformations into perceivable color changes, enabling in situ visualization and quantification of tiny vertical cell forces with high throughput. It enabled long-term, cross-scale monitoring from subcellular focal adhesions to tissue-level cell sheets and aggregates.
目前用于可视化和量化细胞力的技术在活细胞成像、通量和多尺度分析方面存在局限性，这阻碍了细胞力研究及其实际应用的进展。我们开发了一种光子晶体细胞力显微镜（PCCFM），可在没有参考的情况下高速（约每秒 20 帧）对宽视场（1.3mm×1.0mm，10×物镜图像）上的垂直细胞力进行成像。光子晶体水凝胶基底（PCS）将微纳米变形转化为可感知的颜色变化，实现了微小垂直细胞力的高通量原位可视化和定量。它能够从亚细胞黏附斑到组织水平的细胞片和聚集体进行长期、跨尺度监测。
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