Cao Xinbo, Wang Xuemeng, Ren Yanxin, Sun Yangcun, Yang Zhichao, Ge Jingping, Ping Wenxiang
Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education and Heilongjiang Provincial Key Laboratory of Plant Genetic Engineering and Biological Fermentation Engineering for Cold Region and Key Laboratory of Microbiology, College of Heilongjiang Province and School of Life Sciences, Heilongjiang University, Harbin, China.
Hebei Key Laboratory of Agroecological Safety, Hebei University of Environmental Engineering, Qinhuangdao, China.
Front Microbiol. 2023 Oct 27;14:1228700. doi: 10.3389/fmicb.2023.1228700. eCollection 2023.
Increasing evidence suggests that the pathogenesis of type 2 diabetes mellitus (T2DM) is closely related to the gut microbiota. Polyphenols have been shown to alleviate T2DM, but the effects of L. polyphenols (LPs) on the gut microbiota and metabolites remain elusive. In this study, the inhibitory effects of fermented L. polyphenols (FLPs) and unfermented L. polyphenols (ULPs) on α-amylase and α-glucosidase and the impact of LP on the gut microbiota and metabolites were investigated. Furthermore, the relationship between the two was revealed through correlation analysis. The results showed that ULP and FLP had the highest inhibitory rates against α-amylase and α-glucosidase at 4 mg ml, indicating a strong inhibitory ability. In addition, LP plays a regulatory role in the concentration of short-chain fatty acids (SCFAs) and tends to restore them to their normal levels. LP reversed the dysbiosis of the gut microbiota caused by T2DM, as evidenced by an increase in the abundance of bacterial genera such as , , and and a decrease in the abundance of bacterial genera such as and . Similarly, after LP intervention, the relationships among microbial species became more complex and interconnected. In addition, the correlation between the gut microbiota and metabolites was established through correlation analysis. These further findings clarify the mechanism of action of LP against T2DM and provide a new target for T2DM interventions.
越来越多的证据表明，2型糖尿病（T2DM）的发病机制与肠道微生物群密切相关。多酚已被证明可缓解T2DM，但罗伊氏乳杆菌多酚（LPs）对肠道微生物群和代谢产物的影响仍不清楚。在本研究中，研究了发酵罗伊氏乳杆菌多酚（FLPs）和未发酵罗伊氏乳杆菌多酚（ULPs）对α-淀粉酶和α-葡萄糖苷酶的抑制作用以及LPs对肠道微生物群和代谢产物的影响。此外，通过相关性分析揭示了两者之间的关系。结果表明，ULP和FLP在4mg/ml时对α-淀粉酶和α-葡萄糖苷酶的抑制率最高，表明具有较强的抑制能力。此外，LPs对短链脂肪酸（SCFAs）的浓度起调节作用，并倾向于将其恢复到正常水平。LPs逆转了由T2DM引起的肠道微生物群失调，表现为诸如、和等细菌属的丰度增加，以及诸如和等细菌属的丰度降低。同样，在LPs干预后，微生物物种之间的关系变得更加复杂和相互关联。此外，通过相关性分析建立了肠道微生物群与代谢产物之间的相关性。这些进一步的发现阐明了LPs对抗T2DM的作用机制，并为T2DM干预提供了新的靶点。
