Department of Prosthetic Dentistry, Stomatological Hospital and Dental School of Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai, China; Department of Orthodontics, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine; College of Stomatology, Shanghai Jiao Tong University; National Center for Stomatology; National Clinical Research Center for Oral diseases; Shanghai Key Laboratory of Stomatology; Shanghai Research Institute of Stomatology Shanghai, China.
Department of Prosthetic Dentistry, Stomatological Hospital and Dental School of Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai, China; Department of Oral Surgery, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine; Shanghai Key Laboratory of Stomatology &Shanghai Research Institute of Stomatology; National Clinical Research Center of Stomatology, Shanghai, China.
Biomed Pharmacother. 2023 Dec 31;169:115907. doi: 10.1016/j.biopha.2023.115907. Epub 2023 Nov 18.
The lack of a functional vascular supply has been identified as a major challenge limiting the clinical introduction of stem cell-based bone tissue engineering (BTE) for the repair of large-volume bone defects (LVBD). Various approaches have been explored to improve the vascular supply in tissue-engineered constructs, and the development of strategies that could effectively induce the establishment of a functional vascular supply has become a major goal of BTE research. One of the state-of-the-art methods is to incorporate both angiogenic and osteogenic cells in co-culture systems. This review clarifies the key concepts involved, summarises the cell types and models used to date, and systematically evaluates their performance. We also discuss the cell-to-cell communication between these two cell types and the strategies explored in BTE constructs with angiogenic and osteogenic cells to optimise their functions. In addition, we outline unresolved issues and remaining obstacles that need to be overcome for further development in this field and eventual successful repair of LVBD.
血管供应的缺乏已被确定为限制基于干细胞的骨组织工程（BTE）在修复大体积骨缺损（LVBD）方面临床应用的主要挑战。已经探索了各种方法来改善组织工程构建体中的血管供应，并且开发能够有效诱导功能性血管供应建立的策略已成为 BTE 研究的主要目标。最先进的方法之一是将血管生成和成骨细胞合并到共培养系统中。这篇综述阐明了所涉及的关键概念，总结了迄今为止使用的细胞类型和模型，并对其性能进行了系统评估。我们还讨论了这两种细胞类型之间的细胞间通讯，以及在具有血管生成和成骨细胞的 BTE 构建体中探索的策略，以优化它们的功能。此外，我们概述了该领域进一步发展和最终成功修复 LVBD 所需要克服的未解决问题和剩余障碍。
