State Key Laboratory of Digital Medical Engineering, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital, School of Life Sciences and Technology, Advanced Institute for Life and Health, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, 210096, China.
Department of Rheumatology and Immunology, Nanjing Drum Tower Hospital, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China.
Adv Sci (Weinh). 2024 Jan;11(1):e2305215. doi: 10.1002/advs.202305215. Epub 2023 Nov 20.
Noise-induced hearing loss (NIHL) is a common outcome of excessive reactive oxygen species in the cochlea, and the targeted delivery of antioxidants to the inner ear is a potential therapeutic strategy. In this paper, a novel natural biomaterials-derived multifunctional delivery system using silk fibroin-polydopamine (PDA)-composited inverse opal microcarriers (PDA@SFMCs) is presented for inner ear drug delivery and NIHL therapy. Due to their large specific surface area and interpenetrating nanochannels, PDA@SFMCs can rapidly load active biomolecules making them a convenient medium for the storage and delivery of such molecules. In addition, surface modification of PDA enables the microcarriers to remain in the round window niche, thus facilitating the precise local and directed delivery of loaded drugs. Based on these features, it is demonstrated here that n-acetylcysteine-loaded silk microcarriers have satisfactory antioxidant properties on cells and can successfully prevent NIHL in guinea pigs. These results indicate that the natural multifunctional silk microcarriers are promising agents for local inner ear drug delivery in the clinic.
噪声性听力损失（NIHL）是耳蜗中过量活性氧物质的常见后果，而将抗氧化剂靶向递送至内耳是一种有潜力的治疗策略。在本文中，提出了一种使用丝素蛋白-聚多巴胺（PDA）复合的反蛋白石微载体（PDA@SFMCs）的新型天然生物材料衍生多功能递药系统，用于内耳药物递送和 NIHL 治疗。由于其具有较大的比表面积和相互贯穿的纳米通道，PDA@SFMCs 可以快速负载活性生物分子，使其成为此类分子存储和递送的便捷介质。此外，PDA 的表面修饰使微载体能够保留在圆窗龛中，从而有利于负载药物的精确局部和靶向递送。基于这些特性，本文证明了载有 N-乙酰半胱氨酸的丝素微载体对细胞具有令人满意的抗氧化性能，并能成功预防豚鼠的 NIHL。这些结果表明，天然多功能丝素微载体有望成为临床局部内耳药物递送的新型载体。
