Department of Cerebrovascular Surgery, Engineering Technology Research Center of Education Ministry of China, Neurosurgery Center, Zhujiang Hospital of Southern Medical University, Guangzhou 510280, Guangdong, China.
School of Life Science and Engineering, Foshan University, Foshan 528000, Guangdong, China.
Carbohydr Polym. 2024 Jan 15;324:121543. doi: 10.1016/j.carbpol.2023.121543. Epub 2023 Nov 2.
Diabetic wounds pose a significant challenge due to their susceptibility to bacterial infection in a high-glucose environment, which impedes the wound healing process. To address this issue, there is a pressing need to develop suitable hydrogels that can promote the regeneration of diabetic wounds in clinical practice. In this study, we designed and fabricated a highly stretchable, adhesive, transparent, and antibacterial hydrogel through a one-pot radical polymerization of N-[Tris (hydroxymethyl) methyl] acrylamide (THMA) and acrylic acid (AA), and with chitosan and the antibiotic tobramycin as the dynamic physical crosslinkers. The copolymer contains a large number of carboxyl and hydroxyl groups, which can form an interpenetrating network structure with chitosan and tobramycin through multiple dynamic non-covalent bonds. This hydrogel exhibited over 1600 % elongation through an energy dissipation mechanism and strong adhesion to various surfaces without any chemical reaction. In vivo, studies conducted on a staphylococcus aureus-infected full-thickness diabetic skin wound model demonstrated that the hydrogel loaded with tobramycin as one of the crosslinkers had a long-lasting antibacterial activity and effectively accelerated wound healing. Therefore, the antibiotic-loaded adhesive hydrogel we proposed holds great promise as a treatment for bacteria-infected diabetic wounds.
糖尿病伤口由于在高糖环境中容易受到细菌感染，阻碍了伤口愈合过程，因此构成了重大挑战。为了解决这个问题，迫切需要开发合适的水凝胶，以便在临床实践中促进糖尿病伤口的再生。在这项研究中，我们通过 N-[三（羟甲基）甲基]丙烯酰胺（THMA）和丙烯酸（AA）的一锅自由基聚合，并使用壳聚糖和抗生素妥布霉素作为动态物理交联剂，设计和制造了一种高拉伸性、高粘性、透明和抗菌的水凝胶。共聚物含有大量的羧基和羟基，可通过多种动态非共价键与壳聚糖和妥布霉素形成互穿网络结构。这种水凝胶通过能量耗散机制实现了超过 1600%的伸长率，并具有很强的粘性，可与各种表面牢固粘合，无需任何化学反应。在体内，对金黄色葡萄球菌感染的全厚糖尿病皮肤伤口模型进行的研究表明，作为交联剂之一负载妥布霉素的水凝胶具有持久的抗菌活性，并能有效加速伤口愈合。因此，我们提出的载抗生素的粘性水凝胶有望成为治疗细菌感染性糖尿病伤口的一种方法。
