Ren Kai, Ke Xiang, Zhang Miao, Ding Yuan, Wang Hao, Chen Hong, Xie Jing, Li Jianshu
College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, P.R. China.
State Key Laboratory of Oral Diseases, West Chin Hospital of Stomatology, Sichuan University, Chengdu 610041, P.R. China.
BME Front. 2024 Oct 2;5:0059. doi: 10.34133/bmef.0059. eCollection 2024.
Skin wound exposed to complex external environment for a long time is highly susceptible to bacterial infection. This work designs a Janus adhesive dual-layer hydrogel containing in situ silver nanoparticles (named PSAP/DXP@AgNPs) with integrated attack and defense to simultaneously kill the existing bacteria and prevent foreign bacterial contamination. The current gauze dressing fixed by tape fails to well fit at skin wound and lacks intrinsic antibacterial property, making it highly prone to causing secondary infection. Moreover, foreign bacteria may contaminate the wound dressing during use, further increasing the risk of secondary infection. In this work, a Janus adhesive dual-layer PSAP/DXP@AgNPs hydrogel is prepared by sequentially building the PSAP gel layer containing zwitterionic poly(sulfobetaine methacrylamide) (PSBMA) on the DXP@AgNPs gel layer containing in situ catechol-reduced AgNPs. The flexible PSAP/DXP@AgNPs can adapt shape change of skin and adhere to skin tissue with interfacial toughness of 153.38 J m relying on its DXP@AgNPs layer, which is beneficial to build favorable fit. The in situ reduced AgNPs released from the DXP@AgNPs layer of PSAP/DXP@AgNPs exhibit obvious antibacterial effects against and , with antibacterial rates of 99% and 88%, respectively. Meanwhile, the hydrated PSAP layer of PSAP/DXP@AgNPs containing PSBMA is able to prevent the bacterial contamination, decreasing the risk of secondary infection. Besides, cell experiments demonstrate that PSAP/DXP@AgNPs is biocompatible. The PSAP/DXP@AgNPs hydrogel with integrated attack and defense simultaneously possessing bacteria-killing and bacteria-antifouling properties is a potential alternative in treating infected skin wound.
长期暴露于复杂外部环境的皮肤伤口极易受到细菌感染。本研究设计了一种含有原位银纳米颗粒的Janus粘附双层水凝胶（命名为PSAP/DXP@AgNPs），具有综合攻防能力，可同时杀灭现有细菌并防止外来细菌污染。目前用胶带固定的纱布敷料不能很好地贴合皮肤伤口，且缺乏内在抗菌性能，极易导致二次感染。此外，在使用过程中外来细菌可能会污染伤口敷料，进一步增加二次感染的风险。在本研究中，通过在含有原位儿茶酚还原银纳米颗粒的DXP@AgNPs凝胶层上依次构建含有两性离子聚（甲基丙烯酰基磺酸甜菜碱）（PSBMA）的PSAP凝胶层，制备了Janus粘附双层PSAP/DXP@AgNPs水凝胶。柔性的PSAP/DXP@AgNPs能够适应皮肤的形状变化，并依靠其DXP@AgNPs层以153.38 J/m的界面韧性粘附于皮肤组织，这有利于形成良好的贴合。从PSAP/DXP@AgNPs的DXP@AgNPs层释放的原位还原银纳米颗粒对金黄色葡萄球菌和大肠杆菌表现出明显的抗菌效果，抗菌率分别为99%和88%。同时，含有PSBMA的PSAP/DXP@AgNPs的水合PSAP层能够防止细菌污染，降低二次感染的风险。此外，细胞实验表明PSAP/DXP@AgNPs具有生物相容性。具有综合攻防能力、同时具备杀菌和抗细菌污染性能的PSAP/DXP@AgNPs水凝胶是治疗感染皮肤伤口的一种潜在替代品。
