State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan, Hubei, China.
Department of Engineering Mechanics, School of Civil Engineering, Wuhan University, Wuhan, Hubei, China.
J Dent Res. 2024 Jan;103(1):42-50. doi: 10.1177/00220345231205492. Epub 2023 Nov 22.
Biomimetic mineralization of dentin collagen appears to be a promising strategy to optimize dentin bonding durability. However, traditional postbonding mineralization strategies based on Ca/P ion release still have some drawbacks, such as being time-consuming, having a spatiotemporal mismatch, and having limited intrafibrillar minerals. To tackle these problems, a prebonding rapid intrafibrillar mineralization strategy was developed in the present study. Specifically, polyacrylic acid-stabilized amorphous calcium fluoride (PAA-ACF) was found to induce rapid intrafibrillar mineralization of the single-layer collagen model and dentin collagen at just 1 min and 10 min, as identified by transmission electron microscopy, scanning electron microscopy, and atomic force microscopy. This strategy has also been identified to strengthen the mechanical properties of demineralized dentin within a clinically acceptable timeframe. Significantly, the bonding strength of the PAA-ACF-treated groups outperformed the control group irrespective of aging modes. In addition, the endogenous matrix metalloproteinases as well as exogenous bacterial erosion were inhibited, thus reducing the degradation of dentin collagen. High-quality integration of the hybrid layer and the underlying dentin was also demonstrated. On the basis of the present results, the concept of "prebonding rapid intrafibrillar mineralization" was proposed. This user-friendly scheme introduced PAA-ACF-based intrafibrillar mineralization into dentin bonding for the first time. As multifunctional primers, PAA-ACF precursors have the potential to shed new light on prolonging the service life of adhesive restorations, with promising significance.
牙本质胶原的仿生矿化似乎是优化牙本质粘接耐久性的一种很有前途的策略。然而，基于 Ca/P 离子释放的传统粘接后矿化策略仍然存在一些缺点，例如耗时、时空不匹配以及纤维内矿物质有限。为了解决这些问题，本研究提出了一种粘接前快速纤维内矿化策略。具体来说，发现聚丙烯酸稳定的无定形氟化钙（PAA-ACF）可诱导单层胶原模型和牙本质胶原在 1 分钟和 10 分钟内快速纤维内矿化，这可通过透射电子显微镜、扫描电子显微镜和原子力显微镜来识别。该策略还被确定可在临床可接受的时间内增强脱矿牙本质的机械性能。值得注意的是，PAA-ACF 处理组的粘接强度无论老化模式如何均优于对照组。此外，内源性基质金属蛋白酶和外源性细菌侵蚀被抑制，从而减少牙本质胶原的降解。还证明了混合层和下面的牙本质的高质量整合。基于本研究结果，提出了“粘接前快速纤维内矿化”的概念。该方案将基于 PAA-ACF 的纤维内矿化首次引入牙本质粘接中，作为多功能底漆，PAA-ACF 前体有可能为延长粘接修复体的使用寿命提供新的思路，具有重要的意义。
