Department of Biochemistry, Periyar University, Salem, Tamil Nadu, 636011, India.
Department of Environmental Science, Periyar University, Salem, Tamil Nadu, 636011, India.
Urolithiasis. 2023 Nov 22;52(1):7. doi: 10.1007/s00240-023-01503-w.
The present study was designed to evaluate the antiurolithiatic effect of PHYMIN-22 against ethylene glycol-induced urolithiasis in rats. Healthy Albino male rats with 200-230 g body weight were randomly divided into five groups, each with 5 animals, control group, EG group (0.75%), PHYMIN-22 treatment group (0.75% EG 14 days and 100 mg/kg PHYMIN-22 next 14 days), PHYMIN-22 drug control group (100 mg/kg) and cystone treatment group (0.75% EG 14 days and 750 mg/kg cystone next 14 days). Biochemical testing was adopted for measuring the blood and urine parameters, as well as the level of antioxidants including superoxide dismutase (SOD), Catalase (Cat), Glutathione peroxidase (GPx) and glutathione (GSH) in kidney tissues. Hematoxylin and eosin (HE) staining was utilized to observe the histopathological changes in the kidney tissue. End of the experiment the PHYMIN-22 treatment reduced the urine and serum calcium (p < 0.01; p < 0.01), oxalate (p < 0.01; p < 0.01), phosphate (p < 0.01; p < 0.01), uric acid (p < 0.001; p < 0.001), protein (p < 0.001; p < 0.001), and creatinine (p < 0.001; p < 0.001) respectively, serum indicators ALT (p < 0.001) and AST (p < 0.001) level and non-enzymic antioxidant GSH (p < 0.001) compared to EG induced urolithiasis animals (Diseased control group). PHYMIN-22 treatment significantly increased urine volume, pH, and body weight, and antioxidants include CAT (p < 0.001; p < 0.001), SOD (p ˃ 0.05; p < 0.05), and GPX (p < 0.01; p < 0.001) compared to Diseased control group animals. The effect of PHYMIN-22 on EG-induced urolithiasis animals could be by improving kidney function, normalizing the urine and serum parameters, maintaining the kidney antioxidants, eliminating crystal deposition, and excretion of unwanted ions from the kidney and urinary tract.
本研究旨在评估 PHYMIN-22 对乙二醇诱导的大鼠尿石症的抗石作用。将 200-230g 体重的健康雄性白化大鼠随机分为五组，每组 5 只，分别为对照组、EG 组（0.75%）、PHYMIN-22 治疗组（0.75% EG 14 天和 100mg/kg PHYMIN-22 接下来 14 天）、PHYMIN-22 药物对照组（100mg/kg）和 cystone 治疗组（0.75% EG 14 天和 750mg/kg cystone 接下来 14 天）。采用生化检测法测定血液和尿液参数，以及肾组织中超氧化物歧化酶（SOD）、过氧化氢酶（Cat）、谷胱甘肽过氧化物酶（GPx）和谷胱甘肽（GSH）等抗氧化剂的水平。采用苏木精和伊红（HE）染色观察肾组织的组织病理学变化。实验结束时，PHYMIN-22 治疗组降低了尿钙（p<0.01;p<0.01）、尿草酸（p<0.01;p<0.01）、尿磷酸盐（p<0.01;p<0.01）、尿尿酸（p<0.001;p<0.001）、尿蛋白（p<0.001;p<0.001）和血清肌酐（p<0.001;p<0.001），与 EG 诱导的尿石症动物（疾病对照组）相比，血清指标 ALT（p<0.001）和 AST（p<0.001）水平以及非酶抗氧化剂 GSH（p<0.001）水平。PHYMIN-22 治疗组显著增加了尿量、pH 值和体重，CAT（p<0.001;p<0.001）、SOD（p>0.05;p<0.05）和 GPX（p<0.01;p<0.001）等抗氧化剂水平均高于疾病对照组动物。PHYMIN-22 对 EG 诱导的尿石症动物的作用可能是通过改善肾功能、使尿液和血清参数正常化、维持肾脏抗氧化剂、消除晶体沉积以及从肾脏和泌尿道中排出不需要的离子来实现的。
