Department of Biochemistry, Bahauddin Zakariya University, Multan, 60800, Pakistan.
HEJ Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, 75270, Pakistan.
Inflammopharmacology. 2024 Apr;32(2):1333-1351. doi: 10.1007/s10787-023-01390-y. Epub 2023 Nov 23.
Biocompatible anti-inflammatory lignin-capped Ag (LCAg) nanoparticles (NPs) were synthesized for the delivery of galloyl β-sitosterol (Galloyl-BS). β-Sitosterol (BS) is effective against inflammatory responses, like cancer-induced inflammations. BS was modified via gallic acid esterification to enhance its anti-inflammatory potential. LCAg NPs were synthesized by a green method and loaded with galloyl-BS. For comparison, pure BS was also loaded onto LCAg NPs in a separate assembly. The antioxidant potential of Galloyl-BS was greater (IC 177 µM) than pure BS. Materials were characterized by FT-IR, SEM, XRD, and Zeta potential. Using UV-Vis spectroscopy, drug release experiments were performed by varying pH, time, concentration, and temperature. Maximum drug release was observed after 18 h at pH 6 and 40 °C. Galloyl-BS showed improved drug loading efficiency, release %age, and antioxidant activity compared to pure BS when loaded onto LCAg NPs. DLCAg exhibited excellent anti-inflammatory activity in rat models. These findings indicate that galloyl-BS (drug)-loaded LCAg (DLCAg) NPs have the potential as an anti-inflammatory agent without any prior release and scavenging in normal cells.
生物相容型抗炎木质素封端银（LCAg）纳米粒子（NPs）被合成用于递送没食子酰基 β-谷甾醇（Galloyl-BS）。β-谷甾醇（BS）对炎症反应有效，如癌症引起的炎症。BS 通过没食子酸酯化进行修饰，以增强其抗炎潜力。LCAg NPs 通过绿色方法合成，并负载 Galloyl-BS。为了比较，纯 BS 也被分别组装到 LCAg NPs 上。Galloyl-BS 的抗氧化潜力更大（IC 177 µM）比纯 BS。材料通过傅里叶变换红外光谱（FT-IR）、扫描电子显微镜（SEM）、X 射线衍射（XRD）和 Zeta 电位进行了表征。通过紫外可见光谱，通过改变 pH 值、时间、浓度和温度进行了药物释放实验。在 pH 6 和 40°C 下 18 小时后观察到最大药物释放。与纯 BS 相比，当负载到 LCAg NPs 上时，Galloyl-BS 显示出更高的载药效率、释放百分比和抗氧化活性。DLCAg 在大鼠模型中表现出优异的抗炎活性。这些发现表明，载药 LCAg（DLCAg） NPs 有望成为一种抗炎剂，而不会在正常细胞中预先释放和清除。
