Laboratory of Applied Microbiology and Genetics of Microorganisms, Pará State University, Tv. Perebebuí, 2623, Marco, Belém, PA, Brazil.
Braz J Microbiol. 2024 Mar;55(1):455-469. doi: 10.1007/s42770-023-01178-w. Epub 2023 Nov 27.
Composting is a natural process of decomposition of organic matter that occurs by the action of microorganisms such as fungi, bacteria, and actinobacteria. The actinobacteria are present throughout the process due to their resistance to different environmental conditions. They are Gram-positive, filamentous bacteria with a high capacity for producing secondary metabolites of biotechnological importance. Thus, the objective of this work was to isolate and characterize actinobacteria from industrial composting soil of oil palm (Elaeis guineensis) in the municipality of Igarapé-Açu, Pará. Ten samples of the material were collected and seeded on soy tryptone agar, Reasoner's 2A agar, and Columbia agar, using the serial dilution technique. For morphological characterization of the strains, Gram staining and microculture were performed, and for biochemical characterization, the motility, triple sugar iron, Simmons citrate, maltose, phenylalanine, catalase, and DNAse tests were performed. It was observed that compost actinobacteria have a great diversity in morphological and metabolic production, which may be associated with the substrate and cultivation conditions. Therefore, palm oil compost material represents a rich source of bacterial biodiversity, bringing new perspectives for the bioprospecting of actinobacteria of biotechnological importance in little explored environments.
堆肥是有机物质在真菌、细菌和放线菌等微生物作用下分解的自然过程。由于放线菌能够抵抗不同的环境条件,因此它们存在于整个过程中。放线菌是革兰氏阳性、丝状细菌,具有产生具有生物技术重要性的次生代谢物的高能力。因此,这项工作的目的是从帕拉州伊加拉佩-阿苏市的油棕(Elaeis guineensis)工业堆肥土壤中分离和鉴定放线菌。采集了十份材料样本,并使用连续稀释技术在大豆胰蛋白胨琼脂、雷纳二氏 2A 琼脂和哥伦比亚琼脂上进行接种。为了对菌株进行形态学特征描述,进行了革兰氏染色和微培养,而为了进行生化特征描述,进行了运动性、三糖铁、西蒙斯枸橼酸盐、麦芽糖、苯丙氨酸、过氧化氢酶和 DNA 酶测试。观察到堆肥放线菌在形态和代谢产物方面具有很大的多样性,这可能与底物和培养条件有关。因此,棕榈油堆肥材料代表了细菌生物多样性的丰富来源,为在探索较少的环境中寻找具有生物技术重要性的放线菌提供了新的视角。