Zhang Yanyan, Li Qian, Wang Zhongxing, Dong Yi, Yi Dan, Wu Tao, Wang Lei, Zhao Di, Hou Yongqing
Engineering Research Center of Feed Protein Resources on Agricultural By-Products, Ministry of Education, Wuhan Polytechnic University, Wuhan, China.
Front Vet Sci. 2023 Mar 30;10:1098579. doi: 10.3389/fvets.2023.1098579. eCollection 2023.
The effects of cinnamaldehyde, carvacrol and thymol complex (CCT) on the growth performance and intestinal function of piglets challenged with lipopolysaccharide (LPS) were determined. Colistin sulphate (CS) was as a positive control.
Piglets ( = 24, 32 days of age) were allocated to four treatments: Control group (fed basal diet), LPS group (fed basal diet), CS+LPS group (fed basal diet + 50 mg/kg CS), and CCT+LPS group (fed basal diet + 50 mg/kg CCT).
Results showed that diarrhea rates of piglets were significantly reduced by CCT and CS supplementation respectively. Further research showed that CS supplementation tended to improve the intestinal absorption function in LPS-challenged piglets. Moreover, CS supplementation significantly reduced the contents of cortisol in blood and malondialdehyde in the duodenum and the activities of inducible nitric oxide synthase in the duodenum and ileum and total nitric oxide synthase in the ileum in LPS-challenged piglets. CS supplementation significantly increased the activities of sucrase in the ileum and myeloperoxidase in the jejunum in LPS-challenged piglets. CS supplementation significantly alleviated the reduced mRNA levels of immune-related genes (IL-4, IL-6, IL-8, IL-10) in mesenteric lymph nodes and jejunum and mucosal growth-related genes (IGF-1, mTOR, ALP) in LPS-challenged piglets. These results suggested that CS supplementation improved the intestinal function in LPS-challenged piglets by improving intestinal oxidative stress, immune stress, and absorption and repair function. However, although CCT supplementation improved oxidative stress by reducing ( < 0.05) the content of malondialdehyde and the activity of nitric oxide synthase in the duodenum, CCT supplementation tended to aggravate the intestinal absorption dysfunction in LPS-challenged piglets. Furthermore, compared with the control and LPS groups, CCT supplementation remarkably elevated the content of prostaglandin in plasma and the mRNA levels of pro-inflammatory factor IL-6 in mesenteric lymph nodes and jejunum, and reduced the activity of maltase in the ileum in LPS-challenged piglets. These results suggested that CCT supplementation had a negative effect on intestinal function by altering intestinal immune stress response and reducing disaccharidase activity in LPS-challenged piglets.
Compared to CS, CCT supplementation exhibited a negative effect on intestinal function, suggesting whether CCT can be as an effective feed additive still needs further study.
测定了肉桂醛、香芹酚和百里香酚复合物（CCT）对脂多糖（LPS）攻毒仔猪生长性能和肠道功能的影响。硫酸黏菌素（CS）作为阳性对照。
将24头32日龄仔猪分为4组：对照组（饲喂基础日粮）、LPS组（饲喂基础日粮）、CS+LPS组（饲喂基础日粮+50mg/kg CS）和CCT+LPS组（饲喂基础日粮+50mg/kg CCT）。
结果表明，添加CCT和CS分别显著降低了仔猪腹泻率。进一步研究表明，添加CS有助于改善LPS攻毒仔猪的肠道吸收功能。此外，添加CS显著降低了LPS攻毒仔猪血液中皮质醇含量、十二指肠中丙二醛含量、十二指肠和回肠中诱导型一氧化氮合酶活性以及回肠中总一氧化氮合酶活性。添加CS显著提高了LPS攻毒仔猪回肠中蔗糖酶活性和空肠中髓过氧化物酶活性。添加CS显著缓解了LPS攻毒仔猪肠系膜淋巴结和空肠中免疫相关基因（IL-4、IL-6、IL-8、IL-10）以及黏膜生长相关基因（IGF-1、mTOR、ALP）mRNA水平的降低。这些结果表明，添加CS通过改善肠道氧化应激、免疫应激以及吸收和修复功能，提高了LPS攻毒仔猪的肠道功能。然而，尽管添加CCT通过降低十二指肠中丙二醛含量和一氧化氮合酶活性改善了氧化应激，但添加CCT往往会加重LPS攻毒仔猪的肠道吸收功能障碍。此外，与对照组和LPS组相比，添加CCT显著提高了LPS攻毒仔猪血浆中前列腺素含量、肠系膜淋巴结和空肠中促炎因子IL-6的mRNA水平，并降低了回肠中麦芽糖酶活性。这些结果表明，添加CCT通过改变肠道免疫应激反应和降低LPS攻毒仔猪双糖酶活性，对肠道功能产生负面影响。
与CS相比，添加CCT对肠道功能有负面影响，表明CCT能否作为一种有效的饲料添加剂仍需进一步研究。
