Department of Infectious Diseases, King's College London, London, UK.
Infection Sciences, Synnovis, London, UK.
Int J Syst Evol Microbiol. 2023 Dec;73(12). doi: 10.1099/ijsem.0.006184.
A novel bacterial strain, GSTT-20 was isolated from an infected, prosthetic endovascular graft explanted from a shepherd in London, United Kingdom. This strain was an aerobic, catalase-positive, oxidase-negative, Gram-stain-negative, motile, curved rod. It grew on blood agar, chocolate agar and MacConkey agar incubated at 37 °C in an aerobic environment after 48 h, appearing as yellow, mucoid colonies. Analysis of the complete 16S rRNA gene sequence showed closest similarity to with 99.6 % identity and with 99.5 % identity. Phylogenetic analysis of the 16S rRNA gene sequence and phylogenomic analysis of single nucleotide polymorphisms within 1530 core genes showed GSTT-20 forms a distinct lineage in the genus of the family . DNA-DNA hybridization assays against GSTT-20 were estimated at 32.1 % for and 31.9 % for . Genome similarity based on average nucleotide identity was 87.50 % when comparing GSTT-20 to . Based on these results, the strain represented a novel species for which the name sp. nov. was proposed. The type strain is GSTT-20 (NCTC 14621=CECT 30390).
一株新型细菌 GSTT-20 从一位英国伦敦的感染性、植入式血管移植物中分离出来。该菌株为需氧、过氧化氢酶阳性、氧化酶阴性、革兰氏染色阴性、运动、弯曲的杆状菌。在有氧环境下，该菌于 37°C 孵育 48 小时后在血琼脂、巧克力琼脂和 MacConkey 琼脂上生长，呈黄色、黏液样菌落。16S rRNA 基因序列分析表明，该菌株与相似度最高，相似度为 99.6%，与相似度为 99.5%。16S rRNA 基因序列的系统发育分析和 1530 个核心基因内单核苷酸多态性的系统基因组分析表明，GSTT-20 在 科的 属中形成一个独特的分支。GSTT-20 与 和 的 DNA-DNA 杂交测定值分别为 32.1%和 31.9%。当将 GSTT-20 与进行全基因组相似性比较时，基于平均核苷酸同一性的基因组相似性为 87.50%。基于这些结果，该菌株代表了一种新型物种，被提议命名为 。其模式菌株为 GSTT-20（NCTC 14621=CECT 30390）。
