Gavzan Hakimeh, Araghi Atefeh, Marzban Abbasabadi Behrokh, Talebpour Nastaran, Golshahi Hannaneh
Department of Basic Sciences, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran.
Department of Clinical Sciences, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran.
Avicenna J Phytomed. 2023 Sep-Oct;13(5):562-574. doi: 10.22038/AJP.2023.22191.
Depression is a serious mental disorder. Despite numerous medications, there are still limitations in depression treatment. So, herbal medicine has been considered an alternative therapy. This survey evaluated the effects of a Persian herbal formula on mice with chronic unpredictable mild stress (CUMS).
A combination of , , , , , , , and was prepared. Except for the control group, animals were subjected to CUMS for 8 weeks in 5 groups (n=10): CUMS, vehicle (distilled water), herbal formula (0.23 ml/mouse), fluoxetine (20 mg/kg), and bupropion (15 mg/kg). All administrations were performed orally daily for the last 4 weeks. The depression and anxiety-like behaviors were assessed by sucrose preference (SPT), tail suspension (TST), forced swimming (FST), and elevated plus-maze (EPM) tests. Superoxidase-dismutase (SOD) activities in tissues, and serum levels of cortisol, alanine-aminotransferase (ALT), and creatinine were measured. Also, histopathological changes were evaluated.
This formula significantly increased SPT (p<0.001) and decreased immobility time in FST and TST (p<0.01), but it was not effective on EPM vs. CUMS mice. The herbal formula did not change the serum level of creatinine or ALT, but insignificantly reduced cortisol vs. CUMS and vehicle groups. SOD activity increased in the brain vs. vehicle group (p<0.05). There were no changes in histological examination.
The herbal formula improved depression-like behaviors which are possibly related to its anti-oxidative effect on the brain. Also, it did not cause any negative changes in the biochemical and histopathological analysis.
抑郁症是一种严重的精神障碍。尽管有多种药物，但抑郁症治疗仍存在局限性。因此，草药已被视为一种替代疗法。本研究评估了一种波斯草药配方对慢性不可预测轻度应激（CUMS）小鼠的影响。
制备了由[具体成分未给出]组成的配方。除对照组外，将动物分为5组（每组n = 10），进行为期8周的CUMS处理：CUMS组、赋形剂（蒸馏水）组、草药配方组（0.23 ml/只小鼠）、氟西汀组（20 mg/kg）和安非他酮组（15 mg/kg）。在最后4周，所有给药均每日经口进行。通过蔗糖偏好试验（SPT）、悬尾试验（TST）、强迫游泳试验（FST）和高架十字迷宫试验（EPM）评估抑郁和焦虑样行为。测量组织中的超氧化物歧化酶（SOD）活性以及血清皮质醇、丙氨酸转氨酶（ALT）和肌酐水平。此外，评估组织病理学变化。
该配方显著提高了SPT（p < 0.001），并减少了FST和TST中的不动时间（p < 0.01），但与CUMS小鼠相比，对EPM无效。草药配方未改变血清肌酐或ALT水平，但与CUMS组和赋形剂组相比，显著降低了皮质醇水平。与赋形剂组相比，大脑中的SOD活性增加（p < 0.05）。组织学检查无变化。
该草药配方改善了抑郁样行为，这可能与其对大脑的抗氧化作用有关。此外，它在生化和组织病理学分析中未引起任何负面变化。
