Aix Marseille Univ, IRD, AP-HM, SSA, VITROME, Marseille, France.
IHU-Méditerranée Infection, Marseille, France.
Arch Microbiol. 2023 Dec 14;206(1):20. doi: 10.1007/s00203-023-03742-2.
The composition of the vaginal microbiota is known to be influenced by various factors and to be associated with several disorders affecting women's health. Although metagenomics is currently a widely used method for studying the human microbiota, it has certain limitations, such as a lack of information on bacterial viability. It is therefore important to use culture-based methods such as culturomics. Here, we used 35 different culture conditions to comprehensively characterize the vaginal bacterial diversity of a single woman's flora. A total of 206 bacterial species, belonging to six phyla (for a little more than half to Firmicutes, followed mainly by Actinobacteria, Bacteroidetes, and Proteobacteria) and 45 families, and 2 fungal species were cultivated. While several species of lactobacilli have been isolated, a wide variety of other bacteria were also separated, including 65 never reported before in vaginal flora, including a new bacterial species, Porphyromonas vaginalis sp. nov. Extensive culture-based methods are essential to establish a comprehensive, evidence-based repertoire of bacterial viability. If combined with molecular methods, they can provide a much more thorough understanding of the vaginal microbiota and fulfil the unknown part of metagenomic studies.
阴道微生物组的组成已知受多种因素影响，并与影响女性健康的几种疾病有关。尽管宏基因组学目前是研究人类微生物组的一种广泛使用的方法，但它存在一定的局限性，例如缺乏有关细菌活力的信息。因此，使用基于培养的方法（如培养组学）很重要。在这里，我们使用 35 种不同的培养条件来全面描述单个女性菌群的阴道细菌多样性。总共培养出 206 种细菌，属于六个门（略多于一半属于厚壁菌门，主要由放线菌门、拟杆菌门和变形菌门组成）和 45 个科，以及 2 种真菌。虽然分离出了几种乳杆菌，但也分离出了多种其他细菌，包括从未在阴道菌群中报道过的 65 种细菌，包括一种新的细菌物种，阴道普氏菌。广泛的基于培养的方法对于建立全面的、基于证据的细菌活力目录至关重要。如果与分子方法结合使用，它们可以更全面地了解阴道微生物组，并填补宏基因组研究的未知部分。
