通过 culturomics 解析尿微生物群谱揭示肠道中大多为厌氧菌。
Deciphering the Urinary Microbiota Repertoire by Culturomics Reveals Mostly Anaerobic Bacteria From the Gut.
作者信息
Dubourg Grégory, Morand Aurélie, Mekhalif Fatima, Godefroy Raphael, Corthier Alice, Yacouba Abdourahamane, Diakite Ami, Cornu Florent, Cresci Marina, Brahimi Samy, Caputo Aurélia, Lechevallier Eric, Tsimaratos Michel, Moal Valérie, Lagier Jean-Christophe, Raoult Didier
机构信息
IRD, AP-HM, Microbes, Evolution, Phylogeny and Infection (MEPHI), IHU Méditerranée Infection, Aix-Marseille University, Marseille, France.
Pédiatrie Spécialisée et Médecine Infantile, Hôpital de la Timone, AP-HM, Marseille, France.
出版信息
Front Microbiol. 2020 Oct 16;11:513305. doi: 10.3389/fmicb.2020.513305. eCollection 2020.
Human urine was considered sterile for a long time. However, 416 species have been previously cultured, including only 40 anaerobic species. Here, we used culturomics, particularly those targeting anaerobes, to better understand the urinary microbiota. By testing 435 urine samples, we isolated 450 different bacterial species, including 256 never described in urine of which 18 were new species. Among the bacterial species identified, 161 were anaerobes (35%). This study increased the known urine repertoire by 39%. Among the 672 bacterial species isolated now at least once from urine microbiota, 431 (64.1%) were previously isolated from gut microbiota, while only 213 (31.7%) were previously isolated from vagina. These results suggest that many members of the microbiota in the urinary tract are in fact derived from the gut, and a paradigm shift is thus needed in our understanding.
长期以来，人们认为人类尿液是无菌的。然而，此前已培养出416种细菌，其中只有40种是厌氧菌。在此，我们运用 culturomics技术，尤其是针对厌氧菌的技术，以更好地了解泌尿微生物群。通过检测435份尿液样本，我们分离出450种不同的细菌物种，其中包括256种从未在尿液中被描述过的物种，其中18种为新物种。在鉴定出的细菌物种中，161种是厌氧菌（占35%）。这项研究使已知的尿液微生物种类增加了39%。在目前至少从尿液微生物群中分离出一次的672种细菌物种中，431种（64.1%）此前是从肠道微生物群中分离出来的，而只有213种（31.7%）此前是从阴道中分离出来的。这些结果表明，泌尿道微生物群的许多成员实际上源自肠道，因此我们的理解需要范式转变。
Zotero 插件