Department of Microbiology, The University of Tennessee, Knoxville, Tennessee, United States of America.
PLoS One. 2023 Dec 15;18(12):e0295257. doi: 10.1371/journal.pone.0295257. eCollection 2023.
Research into marine iron cycles and biogeochemistry has commonly relied on the use of chelators (including siderophores) to manipulate iron bioavailability. To test whether a commonly used chelator, desferrioxamine B (DFB) caused effects beyond changing the iron-status of cells, cultures of the environmentally relevant marine heterotrophic bacterium, Ruegeria pomeroyii, were grown in media with different concentrations of iron and/or DFB, resulting in a gradient of iron availability. To determine how cells responded, transcriptomes were generated for cells from the different treatments and analyzed to determine how cells reacted to these to perturbations. Analyses were also performed to look for cellular responses specific to the presence of DFB in the culture medium. As expected, cells experiencing different levels of iron availability had different transcriptomic profiles. While many genes related to iron acquisition were differentially expressed between treatments, there were many other genes that were also differentially expressed between different sample types, including those related to the uptake and metabolism of other metals as well as genes related to metabolism of other types of molecules like amino acids and carbohydrates. We conclude that while DFB certainly altered iron availability to cells, it also appears to have had a general effect on the homeostasis of other metals as well as influenced metabolic processes outside of metal acquisition.
海洋铁循环和生物地球化学的研究通常依赖于螯合剂（包括铁载体）的使用来操纵铁的生物利用度。为了测试一种常用的螯合剂，去铁胺 B（DFB）是否会产生改变细胞铁状态以外的影响，我们在含有不同铁浓度和/或 DFB 的培养基中培养了具有环境相关性的海洋异养菌鲁氏不动杆菌的培养物，从而形成了铁可用性的梯度。为了确定细胞如何反应，我们为不同处理的细胞生成了转录组，并进行了分析以确定细胞如何对这些扰动做出反应。还进行了分析，以寻找培养基中存在 DFB 时特定于细胞的反应。正如预期的那样，经历不同铁可用性水平的细胞具有不同的转录组图谱。虽然许多与铁摄取相关的基因在处理之间存在差异表达，但还有许多其他基因在不同样本类型之间也存在差异表达，包括与其他金属的摄取和代谢以及与其他类型分子（如氨基酸和碳水化合物）代谢相关的基因。我们得出的结论是，虽然 DFB 肯定会改变细胞的铁可用性，但它似乎也对其他金属的内稳态产生了普遍影响，并影响了除金属摄取以外的代谢过程。
