Department of Biomedical Engineering, Central Tehran Branch, Islamic Azad University, Tehran, Iran.
Department of Chemistry, Amirkabir University of Technology (AUT), Tehran, Iran.
Int Wound J. 2024 Apr;21(4):e14571. doi: 10.1111/iwj.14571. Epub 2023 Dec 18.
Platelet-rich fibrin (PRF), which is the rich source of growth factors, has been used as an efficient scaffold in tissue engineering and wound healing. In this study, tannic acid as a green cross-linker with different concentrations (0.5%, 1%, 5% and 10%) was used to improve the properties of PRF. The cross-linked gel scaffolds were evaluated by analyses such as scanning electron microscopy, Fourier transform infrared spectroscopy, swelling and degradation, mechanical strength, cell toxicity, cell adhesion and antibacterial test. The results showed that the scaffold structure changes by increasing cross-linker concentration. The swelling rate decreased from 49% to 5% for the samples without the cross-linker and with tannic acid (10%), respectively. The degradation percentage for the cross-linked samples was 8%, which showed a lower degradation rate than the non-cross-linked samples (63%). The mechanical strength of the scaffold with the cross-linker increased up to three times (Young's modulus for the non-cross linked and the cross-linked samples: 0.01 and 0.6 MPa, respectively). Cytotoxicity was not observed up to 10% cross-linker concentration. The cells proliferated well on the cross-linked scaffolds and also showed a good antibacterial effect. In general, tannic acid can improve the physical and mechanical properties of PRF without negatively affecting its biological properties.
富血小板纤维蛋白（PRF）富含生长因子，已被用作组织工程和伤口愈合的有效支架。在这项研究中，使用了不同浓度（0.5%、1%、5%和 10%）的单宁酸作为绿色交联剂来改善 PRF 的性能。通过扫描电子显微镜、傅里叶变换红外光谱、溶胀和降解、机械强度、细胞毒性、细胞黏附和抗菌试验等分析来评估交联凝胶支架。结果表明，随着交联剂浓度的增加，支架结构发生变化。未交联和用单宁酸（10%）交联的样品的溶胀率分别从 49%降至 5%。交联样品的降解百分比为 8%，表明降解速率低于未交联样品（63%）。交联支架的机械强度增加了三倍（未交联和交联样品的杨氏模量分别为 0.01 和 0.6 MPa）。在高达 10%的交联剂浓度下未观察到细胞毒性。细胞在交联支架上很好地增殖，并且还表现出良好的抗菌效果。总的来说，单宁酸可以改善 PRF 的物理和机械性能，而不会对其生物性能产生负面影响。
