Department of Orthodontics, Central Laboratory, National Center for Stomatology, National Engineering Research Center of Oral Biomaterials and Digital Medical Devices Beijing Key Laboratory of Digital Stomatology, Peking University School and Hospital of Stomatology, Beijing, 100081, China.
CAS Center for Excellence in Nanoscience, Beijing Key Laboratory of Micro-nano Energy and Sensor, Beijing Institute of Nanoenergy and Nanosystems, Chinese Academy of Sciences, Beijing, 101400, China.
Small. 2024 May;20(19):e2309230. doi: 10.1002/smll.202309230. Epub 2023 Dec 19.
Bone infection poses a major clinical challenge that can hinder patient recovery and exacerbate postoperative complications. This study has developed a bioactive composite scaffold through the co-assembly and intrafibrillar mineralization of collagen fibrils and zinc oxide (ZnO) nanowires (IMC/ZnO). The IMC/ZnO exhibits bone-like hierarchical structures and enhances capabilities for osteogenesis, antibacterial activity, and bacteria-infected bone healing. During co-cultivation with human bone marrow mesenchymal stem cells (BMMSCs), the IMC/ZnO improves BMMSC adhesion, proliferation, and osteogenic differentiation even under inflammatory conditions. Moreover, it suppresses the activity of Gram-negative Porphyromonas gingivalis and Gram-positive Streptococcus mutans by releasing zinc ions within the acidic infectious microenvironment. In vivo, the IMC/ZnO enables near-complete healing of infected bone defects within the intricate oral bacterial milieu, which is attributed to IMC/ZnO orchestrating M2 macrophage polarization, and fostering an osteogenic and anti-inflammatory microenvironment. Overall, these findings demonstrate the promise of the bioactive scaffold IMC/ZnO for treating bacteria-infected bone defects.
骨感染是一个重大的临床挑战，可能会妨碍患者的康复并加剧术后并发症。本研究通过胶原蛋白纤维和氧化锌（ZnO）纳米线的共组装和纤维内矿化作用，开发了一种具有生物活性的复合支架（IMC/ZnO）。IMC/ZnO 具有类似骨的分级结构，增强了成骨、抗菌活性和细菌感染性骨愈合的能力。在与人骨髓间充质干细胞（BMMSCs）共培养时，即使在炎症条件下，IMC/ZnO 也能提高 BMMSC 的黏附、增殖和成骨分化能力。此外，它通过在酸性感染微环境中释放锌离子来抑制革兰氏阴性牙龈卟啉单胞菌和革兰氏阳性变形链球菌的活性。在体内，IMC/ZnO 可在复杂的口腔细菌环境中实现感染性骨缺损的近乎完全愈合，这归因于 IMC/ZnO 协调 M2 巨噬细胞极化，并促进成骨和抗炎微环境。总的来说，这些发现表明生物活性支架 IMC/ZnO 有望用于治疗细菌感染性骨缺损。
