用于检测从不同来源分离的大肠杆菌产生的热不稳定肠毒素的被动免疫溶血法。
Passive immune hemolysis for detection of heat-labile enterotoxin produced by Escherichia coli isolated from different sources.
作者信息
Serafim M B, Pestana de Castro A F, Lemos dos Reis M H, Trabulsi L R
出版信息
Infect Immun. 1979 Jun;24(3):606-10. doi: 10.1128/iai.24.3.606-610.1979.
Abstract
Fifty-one strains of Escherichia coli isolated from humans, swine, food, and water and identified as enterotoxinogenic by the Y-1 adrenal cell assay, were examined for heat-labile enterotoxin (LT) production by the passive immune hemolysis test. Cholera antitoxin, anti-choleragenoid and anti-LT were used as antisera. Cholera antitoxin was much more potent than anti-choleragenoid and LT antiserum in the detection of LT-positive strains. All strains isolated from pigs and sausage were negative in tests made with LT antiserum. A few strains isolated from humans, food, and water also gave negative results. These data showed that the passive immune hemolysis test is not as efficient as the Y-1 adrenal cell assay in the detection of enterotoxinogenic E. coli strains.
摘要
从人、猪、食物和水中分离出51株大肠杆菌，通过Y-1肾上腺细胞试验鉴定为产肠毒素菌，采用被动免疫溶血试验检测其热不稳定肠毒素（LT）的产生。使用霍乱抗毒素、抗类霍乱原和抗LT作为抗血清。在检测LT阳性菌株方面，霍乱抗毒素比抗类霍乱原和LT抗血清更有效。从猪和香肠中分离出的所有菌株在用LT抗血清进行的试验中均为阴性。从人、食物和水中分离出的一些菌株也得到了阴性结果。这些数据表明，在检测产肠毒素大肠杆菌菌株方面，被动免疫溶血试验不如Y-1肾上腺细胞试验有效。
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