被动免疫溶血法在检测产肠毒素大肠杆菌产生的热不稳定肠毒素中的价值
Value of passive immune hemolysis for detection of heat-labile enterotoxin produced by enterotoxigenic Escherichia coli.
作者信息
Tsukamoto T, Kinoshita Y, Taga S, Takeda Y, Miwatani T
出版信息
J Clin Microbiol. 1980 Dec;12(6):768-71. doi: 10.1128/jcm.12.6.768-771.1980.
Abstract
The method of passive immune hemolysis of Evans and Evans (Infect. Immun. 16:604-609, 1977) for detection of heat-labile enterotoxin produced by enterotoxigenic Escherichia coli was modified. A total of 373 strains of E. coli were tested by this method using materials obtained by treating the cells with polymyxin B and rabbit antiserum against cholera enterotoxin, purified by affinity gel column coupled with purified cholera enterotoxin, in N-hydroxyethylpiperazine-N'-2-ethanesulfonic acid buffer (pH 6.7). The results correlated very well with those obtained in an assay with Chinese hamster ovary cells. It is concluded that passive immune hemolysis is useful as a routine clinical method for identifying E. coli strains that produce heat-labile enterotoxin.
摘要
埃文斯和埃文斯（《感染与免疫》16:604 - 609，1977年）用于检测产肠毒素大肠杆菌产生的热不稳定肠毒素的被动免疫溶血方法被改进。使用多粘菌素B处理细胞获得的材料、经与纯化霍乱肠毒素偶联的亲和凝胶柱纯化的抗霍乱肠毒素兔抗血清，在N - 羟乙基哌嗪 - N'-2 - 乙磺酸缓冲液（pH 6.7）中，用该方法共检测了373株大肠杆菌。结果与用中国仓鼠卵巢细胞进行的检测结果非常吻合。得出的结论是，被动免疫溶血作为一种常规临床方法，对于鉴定产生热不稳定肠毒素的大肠杆菌菌株是有用的。
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