CAS Key Laboratory for Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, China.
Hunan Agricultural University, Changsha, Hunan, China.
NPJ Biofilms Microbiomes. 2020 Feb 14;6(1):8. doi: 10.1038/s41522-020-0117-2.
The microbiota colonizing the root endophytic compartment and surrounding rhizosphere soils contribute to plant growth and health. However, the key members of plant soil and endophytic microbial communities involved in inhibiting or assisting pathogen invasion remain elusive. By utilizing 16S high-throughput sequencing and a molecular ecological network (MEN) approach, we systematically studied the interactions within bacterial communities in plant endophytic compartments (stem and root) and the surrounding soil (bulk and rhizosphere) during bacterial wilt invasion. The endophytic communities were found to be strongly influenced by pathogen invasion according to analysis of microbial diversity and community structure and composition. Endophytic communities of the infected plants were primarily derived from soil communities, as assessed by the SourceTracker program, but with rare migration from soil communities to endophytic communities observed in healthy plants. Soil and endophytic microbiomes from infected plants showed modular topology and greater complexity in network analysis, and a higher number of interactions than those in healthy plants. Furthermore, interactions among microbial members revealed that pathogenic Ralstonia members were positively correlated with several bacterial genera, including Delftia, Stenotrophomonas, Bacillus, Clostridium XlVa, Fontibacillus, Acidovorax, Herminiimonas, and three unclassified bacterial genera, in infected plant roots. Our findings indicated that the pathogen invasion in the rhizosphere and endophytic compartments may be highly associated with bacteria that are normally not detrimental, and sometimes even beneficial, to plants.
定殖于根内共生区和周围根际土壤的微生物群落有助于植物的生长和健康。然而，参与抑制或协助病原体入侵的植物土壤和内生微生物群落的关键成员仍然难以捉摸。本研究利用 16S 高通量测序和分子生态网络（MEN）方法，系统研究了在细菌性萎蔫病入侵过程中植物内生区（茎和根）和周围土壤（体相和根际）内细菌群落的相互作用。根据微生物多样性和群落结构及组成的分析，内生群落受到病原体入侵的强烈影响。通过 SourceTracker 程序评估，感染植物的内生群落主要来源于土壤群落，但在健康植物中观察到从土壤群落向内生群落的罕见迁移。与健康植物相比，感染植物的土壤和内生微生物组在网络分析中表现出模块拓扑结构和更高的复杂性，并且相互作用的数量更多。此外，微生物成员之间的相互作用表明，致病性 Ralstonia 成员与包括 Delftia、Stenotrophomonas、Bacillus、Clostridium XlVa、Fontibacillus、Acidovorax、Herminiimonas 和三个未分类的细菌属在内的几种细菌呈正相关，在感染植物的根部。我们的研究结果表明，根际和内生区的病原体入侵可能与通常对植物无害甚至有益的细菌高度相关。
