Institute of Chemistry, Saint Petersburg State University, 26 Universitetskii pr, 198504 St. Petersburg, Russia.
Nanotechnology Research and Education Centre RAS, Saint Petersburg Academic University, 8/3 Khlopina Street, 194021 St. Petersburg, Russia.
Int J Mol Sci. 2023 Dec 8;24(24):17269. doi: 10.3390/ijms242417269.
Fluorescence of the vast majority of natural opsin-based photoactive proteins is extremely low, in accordance with their functions that depend on efficient transduction of absorbed light energy. However, several recently proposed classes of engineered rhodopsins with enhanced fluorescence, along with the discovery of a new natural highly fluorescent rhodopsin, NeoR, opened a way to exploit these transmembrane proteins as fluorescent sensors and draw more attention to studies on this untypical rhodopsin property. Here, we review the available data on the fluorescence of the retinal chromophore in microbial and animal rhodopsins and their photocycle intermediates, as well as different isomers of the protonated retinal Schiff base in various solvents and the gas phase.
绝大多数天然视蛋白基光活性蛋白的荧光都非常低，这与其功能一致，即依赖于吸收光能的有效转导。然而，最近提出的几类具有增强荧光的工程化视蛋白，以及新型天然高荧光视蛋白 NeoR 的发现，为利用这些跨膜蛋白作为荧光传感器开辟了道路，并使人们更加关注这种非典型视蛋白特性的研究。在这里，我们回顾了微生物和动物视蛋白及其光循环中间体中视黄醛发色团以及不同溶剂和气相中质子化视黄醛席夫碱的不同异构体的荧光的现有数据。
Zotero 插件
