New Insights into Detection of a Dendrobine Compound From a Novel Endophytic Strain and Its Toxicity Against Phytopathogenic Bacteria.

is the only plant that could produce the natural bioactive dendrobine. No other source of dendrobine has been found to date except from and via chemical synthesis. In this study, we aimed to examine the potential fungal endophyte isolated from stem segments using the molecular method and to detect dendrobine compound through high-performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), and liquid chromatography with tandem mass spectrometry (LC-MS/MS) and their metabolite for their antibacterial activity. The potential dendrobine producer strain was recognized as based on molecular DNA sequencing and GenBank databases. The MD33 produced dendrobine and other compounds in a potato dextrose medium (PDM), as confirmed by HPLC retention time peak analysis. The HPLC results revealed that MD33 biomass showed a peak retention time of 5.28 ± 0.2 min, similar to wild stem dendrobine (5.32 ± 0.2 min) and standard chemical reference dendrobine (5.30 ± 0.2 min), indicating the presence of dendrobine in the fungal biomass. Results of GC-MS and LC-MS analysis revealed that MD33 produced the same molecular weight (263 in GC-MS and 264.195 in LC-MS) of dendrobine as compared with standard chemical reference dendrobine and dendrobine. Antibacterial activity data revealed that MD33 produced the strongest bactericidal activity against , , and species, and the diameter of the bacterial growth inhibition zone was 12 ± 0.2, 9 ± 0.2, and 8 ± 0.2 mm, respectively. To the best of our knowledge, this was the first study to investigate as a dendrobine producer, and the results revealed that was directly involved in the potential production of a similar bioactive compound to (dendrobine). In addition, the metabolite exhibited potent antibacterial activity and can be a potential strain for medical and industrial purposes.