小麦中ALKB同源基因家族的全基因组鉴定与特征分析（）。

Genome-wide identification and characterization of ALKB homolog gene family in wheat ().

作者信息

Wang Pengkun, Zhang Tianye, Wu Zechi, Yu Lei, Liao Pingan, Yang Jian, Sun Bingjian

机构信息

The Engineering Research Center for Plant Health Protection Technology in Henan Province, College of Plant Protection, Henan Agricultural University, Zhengzhou, Henan, China.

State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Rural Affairs (MARA), Key Laboratory of Green Plant Protection of Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo, China.

出版信息

Front Plant Sci. 2025 Mar 18;16:1544879. doi: 10.3389/fpls.2025.1544879. eCollection 2025.

DOI:10.3389/fpls.2025.1544879

PMID:40171482

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11959028/

Abstract

INTRODUCTION

N-methyladenosine (mA) is the most prevalent posttranscriptional modification in eukaryotic mRNAs. AlkB homologs (ALKBHs) are involved in plant responses to stress by modulating mA methylation. However, homologous genes in wheat remain largely uncharacterized.

METHODS AND RESULTS

In this study, 30 genes were identified in wheat, and analyzed their physicochemical properties. The phylogenetic analysis allowed the classification of these genes into seven distinct subfamilies. Additionally, their conserved domains, motif compositions, gene structures, chromosomal localization, and synteny, and the predicted cis-acting elements within their promoters were examined. Expression analysis revealed that -5 exhibited the highest expression and its demethylase activity was investigated. Furthermore, -5 was significantly upregulated in response to abscisic acid treatment and cold stress, indicating a positive regulatory trend.

DISCUSSION

In conclusion, this study provides a comprehensive genomic assessment of the gene family and offers a theoretical framework for understanding the role of in the response to abiotic stress in wheat.

摘要

引言

N-甲基腺苷（mA）是真核生物mRNA中最普遍的转录后修饰。AlkB同源物（ALKBHs）通过调节mA甲基化参与植物对胁迫的反应。然而，小麦中的同源基因在很大程度上仍未得到表征。

方法与结果

在本研究中，从小麦中鉴定出30个基因，并分析了它们的理化性质。系统发育分析将这些基因分为七个不同的亚家族。此外，还研究了它们的保守结构域、基序组成、基因结构、染色体定位和共线性，以及其启动子内预测的顺式作用元件。表达分析表明，-5表现出最高的表达，并对其去甲基化酶活性进行了研究。此外，-5在脱落酸处理和冷胁迫下显著上调，表明其具有正调控趋势。